Resources
Plasmid Files

mseCFP

Monomeric super enhanced variant of the cyan fluorescent protein CFP.

 
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 600 400 200 mseCFP End (720) BsrGI (710) TaqII (625) BlpI (622) BsiHKAI (610) BmrI (606) Bpu10I (604) BstYI (500) PfoI * (281) Bts α I (214) BssS α I (181) BsrFI (151) BtgZI (122) EaeI (72) BanI (36) BseRI (31) Start (0) mseCFP 720 bp
End  (720)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TaqII  (625)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsiHKAI  (610)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BmrI  (606)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BtsαI  (214)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsI sites may not be compatible.
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EaeI  (72)
1 site
Y G G C C R R C C G G Y
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
mseCFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
   Segment 1:  
   1 .. 3  =  3 bp
   1 amino acid  =  149.2 Da
Product: monomeric super enhanced variant of CFP
(Matsuda et al., 2008)
mammalian codon-optimized
mseCFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
   Segment 2:  1a  
   4 .. 6  =  3 bp
   1 amino acid  =  117.2 Da
Product: monomeric super enhanced variant of CFP
(Matsuda et al., 2008)
mammalian codon-optimized
mseCFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
   Segment 3:  
   7 .. 720  =  714 bp
   237 amino acids  =  26.7 kDa
Product: monomeric super enhanced variant of CFP
(Matsuda et al., 2008)
mammalian codon-optimized
mseCFP
1 .. 720  =  720 bp
239 amino acids  =  27.0 kDa
3 segments
Product: monomeric super enhanced variant of CFP
(Matsuda et al., 2008)
mammalian codon-optimized
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