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Plasmid Files

pDendra2

Vector for expressing Dendra2 in bacteria.

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pDendra2.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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EcoO109I (4107) AatII (4053) ZraI (4051) XmnI (3730) PvuI (3501) FspI (3353) NmeAIII (3279) BsrFI (3211) BsaI (3192) AhdI (3131) AlwNI (2654) AflIII - PciI (2238) BspQI - SapI (2122) BstAPI (2062) PaeR7I - XhoI (1) PsiI (49) MfeI (59) BseRI (115) BsaBI * (125) BamHI (126) TspMI - XmaI (140) SmaI (142) BclI * (154) EcoNI (156) KasI (285) NarI (286) SfoI (287) PluTI (289) AleI (324) BanII (568) PmlI (587) BstXI (689) BsrGI (838) HindIII (849) BlpI (861) NheI (969) BmtI (973) Bpu10I (994) BspEI (1217) PasI (1453) MscI (1488) NcoI - StyI (1522) XbaI (1825) PfoI (1882) PflFI - Tth111I (1985) AccI (2010) BstZ17I (2011) NdeI (2061) pDendra2 4123 bp
EcoO109I  (4107)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4053)
1 site
G A C G T C C T G C A G
ZraI  (4051)
1 site
G A C G T C C T G C A G
XmnI  (3730)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (3501)
1 site
C G A T C G G C T A G C
FspI  (3353)
1 site
T G C G C A A C G C G T
NmeAIII  (3279)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (3211)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BsaI  (3192)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3131)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2654)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2238)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2238)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2122)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2122)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2062)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T