Resources
Plasmid Files

pDsRed-Express-1

Promoterless reporter vector encoding rapidly maturing DsRed-Express.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pDsRed-Express-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
Download Free Trial Get SnapGene Viewer


AfeI (14) AflIII - PciI (4049) ApaLI (3735) BsaI (3120) PfoI (2906) RsrII (2647) BsrDI (2364) PflFI - Tth111I (2249) PluTI (2134) SfoI (2132) NarI (2131) KasI (2130) BglII (27) PaeR7I - XhoI (31) Eco53kI (36) SacI (38) HindIII (40) EcoRI (47) SalI (57) AccI (58) Acc65I (63) KpnI (67) SacII (70) PspOMI (71) TspMI - XmaI (74) ApaI (75) SmaI (76) BamHI (78) AgeI (84) ZraI (114) AatII (116) FspAI (145) AhdI (276) SbfI (437) BbsI (517) PflMI * (615) BstXI (639) BsgI (676) AleI (724) BssHII (741) NotI (775) XbaI * (785) MfeI (881) HpaI (894) Bts α I (970) AflII (1013) DraIII (1247) SfiI (1906) BspDI * - ClaI * (1971) pDsRed-Express-1 4107 bp
AfeI  (14)
1 site
A G C G C T T C G C G A
AflIII  (4049)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4049)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (3735)
1 site
G T G C A C C A C G T G
BsaI  (3120)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2906)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (2647)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2364)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2249)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2249)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2134)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2132)
1 site
G G C G C C C C G C G G
NarI  (2131)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2130)
1 site
G G C G C C C C G C G G
BglII  (27)
1 site
A G A T C T T C T A G A
PaeR7I  (31)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (31)
1 site
C T C G A G G A G C T C
Eco53kI  (36)
1 site
G A G C T C C T C G A G
SacI  (38)
1 site
G A G C T C C T C G A G
HindIII  (40)
1 site
A A G C T T T T C G A A
EcoRI  (47)
1 site
G A A T T C C T T A A G
SalI  (57)
1 site
G T C G A C C A G C T G
AccI  (58)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (63)
1 site
G G T A C C C C A T G G
KpnI  (67)
1 site
G G T A C C C C A T G G
SacII  (70)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (71)
1 site
G G G C C C C C C G G G
TspMI  (74)
1 site
C C C G G G G G G C C C
XmaI  (74)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (75)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (76)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (78)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
AgeI  (84)
1 site
A C C G G T T G G C C A
ZraI  (114)
1 site
G A C G T C C T G C A G
AatII  (116)
1 site
G A C G T C C T G C A G
FspAI  (145)
1 site
R T G C G C A Y Y A C G C G T R
AhdI  (276)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (437)
1 site
C C T G C A G G G G A C G T C C
BbsI  (517)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (615)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BstXI  (639)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsgI  (676)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AleI  (724)
1 site
C A C N N N N G T G G T G N N N N C A C
BssHII  (741)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NotI  (775)
1 site
G C G G C C G C C G C C G G C G
XbaI  (785)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (881)
1 site
C A A T T G G T T A A C
HpaI  (894)
1 site
G T T A A C C A A T T G
BtsαI  (970)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
AflII  (1013)
1 site
C T T A A G G A A T T C
DraIII  (1247)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (1906)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BspDI  (1971)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1971)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
NeoR/KanR
2003 .. 2797  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
NeoR/KanR
2003 .. 2797  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)
DsRed-Express
97 .. 774  =  678 bp
225 amino acids  =  25.7 kDa
Product: rapidly maturing tetrameric variant of DsRed fluorescent protein
mammalian codon-optimized
DsRed-Express
97 .. 774  =  678 bp
225 amino acids  =  25.7 kDa
Product: rapidly maturing tetrameric variant of DsRed fluorescent protein
mammalian codon-optimized
ori
3405 .. 3993  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
3405 .. 3993  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
1023 .. 1478  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
1023 .. 1478  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
SV40 promoter
1611 .. 1968  =  358 bp
SV40 enhancer and early promoter
SV40 promoter
1611 .. 1968  =  358 bp
SV40 enhancer and early promoter
SV40 poly(A) signal
895 .. 1016  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
895 .. 1016  =  122 bp
SV40 polyadenylation signal
AmpR promoter
1505 .. 1609  =  105 bp
AmpR promoter
1505 .. 1609  =  105 bp
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
MCS
12 .. 89  =  78 bp
multiple cloning site of fluorescent protein plasmids
HSV TK poly(A) signal
3029 .. 3076  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
3029 .. 3076  =  48 bp
herpesvirus thymidine kinase polyadenylation signal
SV40 ori
1819 .. 1954  =  136 bp
SV40 origin of replication
SV40 ori
1819 .. 1954  =  136 bp
SV40 origin of replication
ORF:  97 .. 774  =  678 bp
ORF:  225 amino acids  =  25.7 kDa
ORF:  2818 .. 3267  =  450 bp
ORF:  149 amino acids  =  16.3 kDa
ORF:  2003 .. 2797  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2175 .. 2561  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  25 .. 804  =  780 bp
ORF:  259 amino acids  =  26.3 kDa
ORF:  2312 .. 2848  =  537 bp
ORF:  178 amino acids  =  19.8 kDa
ORF:  3023 .. 3256  =  234 bp
ORF:  77 amino acids  =  8.6 kDa
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps