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Plasmid Files

pDsRed-Express-C1

Vector for fusing rapidly maturing DsRed-Express to the N-terminus of a partner protein.

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pDsRed-Express-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4622) ApaLI (4308) BsaI (3693) PfoI (3479) RsrII (3220) BsrDI (2937) PflFI - Tth111I (2822) PluTI (2707) SfoI (2705) NarI (2704) KasI (2703) EagI (2610) BspDI * - ClaI * (2544) SfiI (2479) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) FspAI (661) AhdI (792) SbfI (953) BbsI (1033) PflMI * (1131) BstXI (1155) BsgI (1192) AleI (1240) BssHII (1257) BglII (1288) PaeR7I - XhoI (1292) Eco53kI (1297) SacI (1299) HindIII (1301) EcoRI (1308) SalI (1318) AccI (1319) Acc65I (1324) KpnI (1328) SacII (1331) PspOMI (1332) TspMI - XmaI (1335) ApaI (1336) SmaI (1337) BamHI (1339) XbaI * (1351) BclI * (1361) MfeI (1454) HpaI (1467) Bts α I (1543) MluI (1590) DraIII (1820) pDsRed-Express-C1 4680 bp
PciI  (4622)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4308)
1 site
G T G C A C C A C G T G
BsaI  (3693)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3479)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3220)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2937)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2822)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PluTI  (2707)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2705)
1 site
G G C G C C C C G C G G
NarI  (2704)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2703)
1 site
G G C G C C C C G C G G
EagI  (2610)
1 site
C G G C C G G C C G G C
BspDI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2544)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2479)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C