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Plasmid Files

pDsRed-Express2-N1

Vector for fusing rapidly maturing and noncytotoxic DsRed-Express2 to the C-terminus of a partner protein.

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pDsRed-Express2-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4631) EcoO109I (3811) BsaI (3702) PfoI (3488) RsrII (3229) BsrDI (2946) PflFI - Tth111I (2831) FspI (2815) PluTI (2716) SfoI (2714) NarI (2713) KasI (2712) BspDI * - ClaI * (2553) StuI (2534) SfiI (2488) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) SpeI (666) AarI (807) AhdI (858) SbfI (1019) PmlI (1041) BbsI (1099) PflMI * (1197) AleI (1306) BssHII (1323) NotI (1357) XbaI * (1367) MfeI (1463) HpaI (1476) Bts α I (1552) AflII (1595) DraIII (1829) pDsRed-Express2-N1 4689 bp
AflIII  (4631)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4631)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3811)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3702)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3488)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3229)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2946)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2831)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2831)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2815)
1 site
T G C G C A A C G C G T
PluTI  (2716)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2714)
1 site
G G C G C C C C G C G G
NarI  (2713)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2712)
1 site
G G C G C C C C G C G G
BspDI  (2553)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2553)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2534)
1 site
A G G C C T T C C G G A
SfiI  (2488)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site