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pDsRed-Monomer-C In-Fusion® Ready

Linearized vector for fusing DsRed-Monomer to the N-terminus of a partner protein.

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pDsRed-Monomer-C In-Fusion Ready.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (3301) ApaLI (2987) EcoO109I (2481) BsaI (2372) PfoI (2158) BstBI (2065) RsrII (1899) BsrDI (1616) PflFI - Tth111I (1501) MscI (1465) PluTI (1386) SfoI (1384) NarI (1383) KasI (1382) EagI (1289) BspDI * - ClaI * (1223) SfiI (1158) AseI (3366) NdeI (3593) SnaBI (3699) NheI (3950) BmtI (3954) AgeI (3959) FspAI (4020) BstEII (4112) AhdI (4151) AvaI - BsoBI (4233) BmeT110I (4234) SbfI (4312) BbsI (4392) BglII (4450) BsrGI (4510) PflMI (4553) AleI (4599) BspEI (4647) AflII (4653) End (4669) Start (0) EcoRI (8) BamHI (18) XbaI * (30) BclI * (40) MfeI (133) HincII - HpaI (146) Bts α I (222) MluI (269) SexAI * (972) pDsRed-Monomer-C In-Fusion® Ready 4669 bp
PciI  (3301)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (2987)
1 site
G T G C A C C A C G T G
EcoO109I  (2481)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (2372)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (2158)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstBI  (2065)
1 site
T T C G A A A A G C T T
RsrII  (1899)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (1616)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (1501)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1501)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
MscI  (1465)
1 site
T G G C C A A C C G G T
PluTI  (1386)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (1384)
1 site
G G C G C C C C G C G G
NarI  (1383)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (1382)
1 site
G G C G C C C C G C G G
EagI  (1289)
1 site
C G G C C G G C C G G C
BspDI  (1223)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (1223)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (1158)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (3366)
1 site
A T T A A T T A A T T A
NdeI  (3593)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (3699)
1 site
T A C G T A A T G C A T
NheI  (3950)
1 site
G C T A G C C G A T C G
BmtI  (3954)
1 site
G C T A G C C G A T C G
AgeI  (3959)
1 site
A C C G G T T G G C C A
FspAI  (4020)
1 site
R T G C G C A Y Y A C G C G T R
BstEII  (4112)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AhdI  (4151)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AvaI  (4233)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (4233)
1 site
C Y C G R G G