pGLO

Bacterial transformation plasmid for regulated expression of GFP.

Sequence Author: Bio-Rad

|Download SnapGene Viewer
No matches
BspDI - ClaI (2) SgrAI (5307) AfeI (5225) BsmBI - Esp3I (5105) PfoI (5103) PflFI - Tth111I (5004) PciI (4748) AlwNI (4339) PsiI (3896) DraIII (3771) AhdI (3423) BsaI (3357) BglI (3305) NmeAIII (3276) AseI (3248) FspI (3200) PvuI (3054) ScaI (2942) NsiI (9) BsaBI (241) AflII (301) EcoRV (386) BssHII (670) NruI (708) PflMI (920) AgeI (1074) BstEII (1077) BsmI (1097) NheI (1345) BmtI (1349) NcoI - StyI (1510) MscI (1515) BsrGI (1620) PaeR7I - PspXI - XhoI (1765) BstBI (1966) EcoRI (2063) Acc65I (2075) KpnI - TspMI - XmaI (2079) SmaI (2081) XbaI (2090) SbfI (2106) BfuAI - BspMI (2109) SphI (2112) KflI - PpuMI (2286) pGLO 5371 bp
BspDI  (2)
1 site
A T C G A T T A G C T A
ClaI  (2)
1 site
A T C G A T T A G C T A
SgrAI  (5307)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AfeI  (5225)
1 site
A G C G C T T C G C G A
BsmBI  (5105)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BsmBI-v2 is an improved version of BsmBI.
Esp3I  (5105)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different Esp3I sites may not be compatible.
PfoI  (5103)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (5004)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5004)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PciI  (4748)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (4339)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PsiI  (3896)
1 site
T T A T A A A A T A T T
DraIII  (3771)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AhdI  (3423)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (3357)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglI  (3305)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
NmeAIII  (3276)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AseI  (3248)
1 site
A T T A A T T A A T T A
FspI  (3200)
1 site
T G C G C A A C G C G T
PvuI  (3054)
1 site
C G A T C G G C T A G C
ScaI  (2942)
1 site
A G T A C T T C A T G A
NsiI  (9)
1 site
A T G C A T T A C G T A
BsaBI  (241)
1 site
G A T N N N N A T C C T A N N N N T A G
AflII  (301)
1 site
C T T A A G G A A T T C
EcoRV  (386)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BssHII  (670)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (708)
1 site
T C G C G A A G C G C T
PflMI  (920)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AgeI  (1074)
1 site
A C C G G T T G G C C A
BstEII  (1077)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsmI  (1097)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NheI  (1345)
1 site
G C T A G C C G A T C G
BmtI  (1349)
1 site
G C T A G C C G A T C G
NcoI  (1510)
1 site
C C A T G G G G T A C C
StyI  (1510)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (1515)
1 site
T G G C C A A C C G G T
BsrGI  (1620)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PaeR7I  (1765)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1765)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1765)
1 site
C T C G A G G A G C T C
BstBI  (1966)
1 site
T T C G A A A A G C T T
EcoRI  (2063)
1 site
G A A T T C C T T A A G
Acc65I  (2075)
1 site
G G T A C C C C A T G G
KpnI  (2079)
1 site
G G T A C C C C A T G G
TspMI  (2079)
1 site
C C C G G G G G G C C C
XmaI  (2079)
1 site
C C C G G G G G G C C C

Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI  (2081)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XbaI  (2090)
1 site
T C T A G A A G A T C T
SbfI  (2106)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (2109)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2109)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (2112)
1 site
G C A T G C C G T A C G
KflI  (2286)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (2286)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
araC
96 .. 974  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
96 .. 974  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   2636 .. 2704  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   2705 .. 3496  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 1:  
   1342 .. 1344  =  3 bp
   1 amino acid  =  149.2 Da
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 2:  1a  
   1345 .. 1347  =  3 bp
   1 amino acid  =  89.1 Da
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
   Segment 3:  
   1348 .. 2061  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Aequoria victoria green fluorescent protein
ori
4104 .. 4692  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
4104 .. 4692  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3538 .. 3993  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3538 .. 3993  =  456 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
araBAD promoter
1120 .. 1285  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
araBAD promoter
1120 .. 1285  =  166 bp
promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction
AmpR promoter
2544 .. 2635  =  92 bp
AmpR promoter
2544 .. 2635  =  92 bp
rrnB T1 terminator
2321 .. 2407  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
2321 .. 2407  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
MCS
2063 .. 2119  =  57 bp
pUC18/19 multiple cloning site
MCS
2063 .. 2119  =  57 bp
pUC18/19 multiple cloning site
ORF:  1342 .. 2061  =  720 bp
ORF:  239 amino acids  =  26.9 kDa
ORF:  2636 .. 3496  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
ORF:  660 .. 908  =  249 bp
ORF:  82 amino acids  =  9.3 kDa
ORF:  5115 .. 109  =  366 bp
ORF:  121 amino acids  =  12.8 kDa
ORF:  2249 .. 2488  =  240 bp
ORF:  79 amino acids  =  8.6 kDa
ORF:  1927 .. 2295  =  369 bp
ORF:  122 amino acids  =  13.6 kDa
ORF:  3100 .. 3366  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  96 .. 1025  =  930 bp
ORF:  309 amino acids  =  35.2 kDa
Click here to try SnapGene

Download pGLO.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.