Resources
Plasmid Files

pGLO

Bacterial transformation plasmid for regulated expression of GFP.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pGLO Sequence and MappGLO.dna
Map and Sequence File   
Sequence Author:  Bio-Rad
Download Free Trial Get SnapGene Viewer

 BspDI - ClaI (2) SgrAI (5307) AfeI (5225) BsmBI (5105) PfoI (5103) PflFI - Tth111I (5004) PciI (4748) AlwNI (4339) PsiI (3896) DraIII (3771) AhdI (3423) BsaI (3357) BglI (3305) NmeAIII (3276) AseI (3248) FspI (3200) PvuI (3054) ScaI (2942) NsiI (9) BsaBI (241) AflII (301) EcoRV (386) BssHII (670) NruI (708) PflMI (920) AgeI (1074) BstEII (1077) BsmI (1097) NheI (1345) BmtI (1349) NcoI - StyI (1510) MscI (1515) BsrGI (1620) PaeR7I - PspXI - XhoI (1765) BstBI (1966) EcoRI (2063) Acc65I (2075) KpnI - TspMI - XmaI (2079) SmaI (2081) XbaI (2090) MCS SbfI (2106) BfuAI - BspMI (2109) SphI (2112) KflI - PpuMI (2286) pGLO 5371 bp
BspDI  (2)
1 site
A T C G A T T A G C T A
ClaI  (2)
1 site
A T C G A T T A G C T A
SgrAI  (5307)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AfeI  (5225)
1 site
A G C G C T T C G C G A
BsmBI  (5105)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
PfoI  (5103)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (5004)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (5004)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
PciI  (4748)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (4339)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PsiI  (3896)
1 site
T T A T A A A A T A T T
DraIII  (3771)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
AhdI  (3423)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (3357)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BglI  (3305)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
NmeAIII  (3276)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AseI  (3248)
1 site
A T T A A T T A A T T A
FspI  (3200)
1 site
T G C G C A A C G C G T
PvuI  (3054)
1 site
C G A T C G G C T A G C
ScaI  (2942)
1 site
A G T A C T T C A T G A
NsiI  (9)
1 site
A T G C A T T A C G T A
BsaBI  (241)
1 site
G A T N N N N A T C C T A N N N N T A G
AflII  (301)
1 site
C T T A A G G A A T T C

The sticky ends produced by AflII are hard to ligate.
EcoRV  (386)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BssHII  (670)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
NruI  (708)
1 site
T C G C G A A G C G C T
PflMI  (920)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
AgeI  (1074)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BstEII  (1077)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
BsmI  (1097)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
NheI  (1345)
1 site
G C T A G C C G A T C G
BmtI  (1349)
1 site
G C T A G C C G A T C G
NcoI  (1510)
1 site
C C A T G G G G T A C C
StyI  (1510)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (1515)
1 site
T G G C C A A C C G G T
BsrGI  (1620)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
PaeR7I  (1765)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1765)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1765)
1 site
C T C G A G G A G C T C
BstBI  (1966)
1 site
T T C G A A A A G C T T
EcoRI  (2063)
1 site
G A A T T C C T T A A G
Acc65I  (2075)
1 site
G G T A C C C C A T G G
KpnI  (2079)
1 site
G G T A C C C C A T G G
TspMI  (2079)
1 site
C C C G G G G G G C C C
XmaI  (2079)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (2081)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
XbaI  (2090)
1 site
T C T A G A A G A T C T
SbfI  (2106)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (2109)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (2109)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SphI  (2112)
1 site
G C A T G C C G T A C G
KflI  (2286)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
PpuMI  (2286)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
araC
96 .. 974  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
araC
96 .. 974  =  879 bp
292 amino acids  =  33.4 kDa
Product: L-arabinose regulatory protein
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   2636 .. 2704  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   2705 .. 3496  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2636 .. 3496  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
   Segment 1:  
   1342 .. 1344  =  3 bp
   1 amino acid  =  149.2 Da
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
   Segment 2:  1a  
   1345 .. 1347  =  3 bp
   1 amino acid  =  89.1 Da
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
   Segment 3:  
   1348 .. 2061  =  714 bp
   237 amino acids  =  26.7 kDa
Product: Aequoria victoria green fluorescent protein
GFP
1342 .. 2061  =  720 bp
239 amino acids  =  26.9 kDa
3 segments
Product: Aequoria victoria green fluorescent protein
ori
4104 .. 4692  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4104 .. 4692  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
3538 .. 3993  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
3538 .. 3993  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
araBAD promoter
1120 .. 1285  =  166 bp
promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction
araBAD promoter
1120 .. 1285  =  166 bp
promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction
AmpR promoter
2544 .. 2635  =  92 bp
AmpR promoter
2544 .. 2635  =  92 bp
rrnB T1 terminator
2321 .. 2407  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
rrnB T1 terminator
2321 .. 2407  =  87 bp
transcription terminator T1 from the E. coli rrnB
gene
MCS
2063 .. 2119  =  57 bp
pUC18/19 multiple cloning site
MCS
2063 .. 2119  =  57 bp
pUC18/19 multiple cloning site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter