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Plasmid Files

pKatushka2S-N

Vector for fusing Katushka2S to the C-terminus of a partner protein.

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pKatushka2S-N.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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AflIII - PciI (4662) EcoO109I (3842) PfoI (3519) RsrII (3260) BsrDI (2977) PflFI - Tth111I (2862) FspI (2846) PluTI (2747) SfoI (2745) NarI (2744) KasI (2743) BspDI * - ClaI * (2584) StuI (2565) BseRI (2562) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) PstI (638) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) AleI (679) BstXI (681) BclI * (701) BsrGI (728) BbsI (986) BsgI (1183) PshAI (1318) BlpI (1383) NotI (1388) XbaI * (1398) MfeI (1494) HpaI (1507) AflII (1626) DraIII (1860) SexAI * (2333) pKatushka2S-N 4720 bp
AflIII  (4662)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4662)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3842)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3519)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3260)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2977)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2862)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2862)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2846)
1 site
T G C G C A A C G C G T
PluTI  (2747)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2745)
1 site
G G C G C C C C G C G G
NarI  (2744)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2743)
1 site
G G C G C C C C G C G G
BspDI  (2584)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2584)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2565)
1 site
A G G C C T T C C G G A
BseRI  (2562)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
BglII  (609)
1 site
A G A T C T T C T A G