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Plasmid Files

pKillerOrange-C

Vector for fusing KillerOrange to the N-terminus of a partner protein.

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pKillerOrange-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PfoI (3524) RsrII (3265) BsrDI (2982) PflFI - Tth111I (2867) FspI (2851) MscI (2831) PluTI (2752) SfoI (2750) NarI (2749) KasI (2748) EagI (2655) BspDI * - ClaI * (2589) StuI (2570) BseRI (2567) SfiI (2524) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) Bpu10I (880) BlpI (946) AarI (964) PmlI (1251) BspEI (1324) BglII (1333) PaeR7I - XhoI (1337) Eco53kI (1342) SacI (1344) HindIII (1346) EcoRI (1353) PstI (1362) SalI (1363) AccI (1364) Acc65I (1369) KpnI (1373) SacII (1376) PspOMI (1377) TspMI - XmaI (1380) ApaI (1381) SmaI (1382) BamHI (1384) XbaI * (1396) BclI * (1406) MfeI (1499) HpaI (1512) Bts α I (1588) MluI (1635) SexAI * (2338) pKillerOrange-C 4725 bp
PfoI  (3524)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3265)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2982)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2867)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2851)
1 site
T G C G C A A C G C G T
MscI  (2831)
1 site
T G G C C A A C C G G T
PluTI  (2752)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2750)
1 site
G G C G C C C C G C G G
NarI  (2749)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2748)
1 site
G G C G C C C C G C G G
EagI  (2655)
1 site
C G G C C G G C C G G C
BspDI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2589)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2570)
1 site
A G G C C T T C C G G A