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Plasmid Files

pKillerOrange-N

Vector for fusing KillerOrange to the C-terminus of a partner protein.

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pKillerOrange-N.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PfoI (3525) RsrII (3266) BsrDI (2983) PflFI - Tth111I (2868) FspI (2852) MscI (2832) PluTI (2753) SfoI (2751) NarI (2750) KasI (2749) BspDI * - ClaI * (2590) StuI (2571) BseRI (2568) SfiI (2525) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) HindIII (622) EcoRI (629) PstI (638) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) Bpu10I (946) BlpI (1012) AarI (1030) PmlI (1317) NotI (1394) XbaI * (1404) MfeI (1500) HpaI (1513) Bts α I (1589) AflII (1632) SexAI * (2339) pKillerOrange-N 4726 bp
PfoI  (3525)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3266)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2983)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2868)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2868)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2852)
1 site
T G C G C A A C G C G T
MscI  (2832)
1 site
T G G C C A A C C G G T
PluTI  (2753)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2751)
1 site
G G C G C C C C G C G G
NarI  (2750)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2749)
1 site
G G C G C C C C G C G G
BspDI  (2590)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2590)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2571)
1 site
A G G C C T T C C G G A
BseRI  (2568)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2525)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
BglII  (609)
1 site
A G A T C T T C T A G A
PaeR7I  (613)
1 site
C T C G A