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Plasmid Files

pKillerRed-C

Vector for fusing KillerRed to the N-terminus of a partner protein.

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pKillerRed-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PfoI (3530) RsrII (3271) BsrDI (2988) PflFI - Tth111I (2873) FspI (2857) MscI (2837) PluTI (2758) SfoI (2756) NarI (2755) KasI (2754) EagI (2661) BspDI * - ClaI * (2595) StuI (2576) BseRI (2573) SfiI (2530) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) Bpu10I (886) AarI (970) PmlI (1257) BspEI (1330) BglII (1339) PaeR7I - XhoI (1343) Eco53kI (1348) SacI (1350) HindIII (1352) EcoRI (1359) PstI (1368) SalI (1369) AccI (1370) Acc65I (1375) KpnI (1379) SacII (1382) PspOMI (1383) TspMI - XmaI (1386) ApaI (1387) SmaI (1388) BamHI (1390) XbaI * (1402) BclI * (1412) MfeI (1505) HpaI (1518) Bts α I (1594) MluI (1641) SexAI * (2344) pKillerRed-C 4731 bp
PfoI  (3530)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3271)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2988)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2873)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2873)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2857)
1 site
T G C G C A A C G C G T
MscI  (2837)
1 site
T G G C C A A C C G G T
PluTI  (2758)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2756)
1 site
G G C G C C C C G C G G
NarI  (2755)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2754)
1 site
G G C G C C C C G C G G
EagI  (2661)
1 site
C G G C C G G C C G G C
BspDI  (2595)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2595)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2576)
1 site
A G G C C T T C C G G A
BseRI  (2573)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SfiI  (2530)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
Bpu10I  (886)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AarI  (970)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the AarI recognition sequence.
Sticky ends from different AarI sites may not be compatible.
After cleavage, AarI can remain bound to DNA and alter its electrophoretic mobility.
PmlI  (1257)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BspEI  (1330)
1 site
T C C G G A A G G C C T
BglII  (1339)
1 site
A G A T C T T C T A G A
PaeR7I  (1343)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1343)
1 site
C T C G A G G A G C T C
Eco53kI  (1348)
1 site
G A G C T C C T C G A G
SacI  (1350)
1 site
G A G C T C C T C G A G
HindIII  (1352)
1 site
A A G C T T T T C G A A
EcoRI  (1359)
1 site
G A A T T C C T T A A G
PstI  (1368)
1 site
C T G C A G G A C G T C
SalI  (1369)
1 site
G T C G A C C A G C T G
AccI  (1370)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1375)
1 site
G G T A C C C C A T G G
KpnI  (1379)
1 site
G G T A C C C C A T G G
SacII  (1382)
1 site
C C G C G G G G C G C C

Eff