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Plasmid Files

pLSSmOrange-C1

Vector for fusing LSSmOrange to the N-terminus of a partner protein.

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pLSSmOrange-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Verkhusha Lab
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PciI (4658) ApaLI (4344) EcoO109I (3838) BsaI (3729) PfoI (3515) RsrII (3256) BsrDI (2973) PflFI - Tth111I (2858) EagI (2646) BspDI * - ClaI * (2580) SfiI (2515) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) FspAI (670) AhdI (801) SbfI (962) BbsI (1042) BstXI (1047) Bpu10I (1108) BsgI (1201) SgrAI (1288) XcmI (1293) BsrGI (1307) BspEI (1315) BglII (1324) PaeR7I - XhoI (1328) Eco53kI (1333) SacI (1335) HindIII (1337) EcoRI (1344) Acc65I (1360) KpnI (1364) SacII (1367) PspOMI (1368) TspMI - XmaI (1371) ApaI (1372) SmaI (1373) BamHI (1375) XbaI * (1387) BclI * (1397) MfeI (1490) HpaI (1503) Bts α I (1579) MluI (1626) DraIII (1856) SexAI * (2329) pLSSmOrange-C1 4716 bp
PciI  (4658)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4344)
1 site
G T G C A C C A C G T G
EcoO109I  (3838)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3729)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3515)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3256)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2973)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2858)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2858)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
EagI  (2646)
1 site
C G G C C G G C C G G C
BspDI  (2580)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2580)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
SfiI  (2515)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
FspAI  (670)
1 site
R T G C G C A Y Y A C G C G T R
AhdI  (801)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (962)
1 site
C C T G C A G G G G A C G T C C
BbsI  (1042)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BstXI  (1047)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
Bpu10I  (1108)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BsgI  (1201)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
SgrAI  (1288)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
XcmI  (1293)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BsrGI  (1307)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BspEI  (1315)
1 site
T C C G G A A G G C C T
BglII  (1324)
1 site
A G A