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Plasmid Files

pPAmCherry-C1

Vector for fusing photoactivatable mCherry to the N-terminus of a partner protein.

To see this sequence with restriction sites, features, and translations, please download
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pPAmCherry-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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PciI (4663) BsaI (3734) PfoI (3520) RsrII (3261) BsrDI (2978) PflFI - Tth111I (2863) FspI (2847) EagI (2651) BspDI * - ClaI * (2585) StuI (2566) SfiI (2520) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) PmlI (701) AhdI (806) SbfI (967) BbsI (1047) PflMI (1051) BbvCI - Bpu10I (1150) BsgI (1206) SgrAI (1293) XcmI (1298) BsrGI (1312) BspEI (1320) BglII (1329) PaeR7I - XhoI (1333) Eco53kI (1338) SacI (1340) HindIII (1342) EcoRI (1349) SalI (1359) AccI (1360) Acc65I (1365) KpnI (1369) SacII (1372) PspOMI (1373) TspMI - XmaI (1376) ApaI (1377) SmaI (1378) BamHI (1380) XbaI * (1392) BclI * (1402) MfeI (1495) HpaI (1508) Bts α I (1584) MluI (1631) DraIII (1861) SexAI * (2334) pPAmCherry-C1 4721 bp
PciI  (4663)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BsaI  (3734)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3520)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3261)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2978)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2863)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2863)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2847)
1 site
T G C G C A A C G C G T
EagI  (2651)
1 site
C G G C C G G C C G G C
BspDI  (2585)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2585)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2566)
1 site
A G G C C T T C C G G A
SfiI  (2520)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
PmlI  (701)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
AhdI  (806)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
SbfI  (967)
1 site
C C T G C A G G G G A C G T C C
BbsI  (1047)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflMI  (1051)
1 site
C C A N N N N N T G G G G T N N N N