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pTagGFP2-C

Vector for fusing TagGFP2 (also known as mTagGFP) to the N-terminus of a partner protein.

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pTagGFP2-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PciI (4664) EcoO109I (3844) BsaI (3735) RsrII (3262) BsrDI (2979) PflFI - Tth111I (2864) FspI (2848) MscI (2828) PluTI (2749) SfoI (2747) NarI (2746) KasI (2745) EagI (2652) BspDI * - ClaI * (2586) StuI (2567) SfiI (2521) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) BmgBI (669) BstEII (786) XmnI (850) Bpu10I (1207) BspEI (1321) BglII (1330) PaeR7I - XhoI (1334) Eco53kI (1339) SacI (1341) HindIII (1343) EcoRI (1350) PstI (1359) SalI (1360) AccI (1361) Acc65I (1366) KpnI (1370) PspOMI (1374) TspMI - XmaI (1377) ApaI (1378) SmaI (1379) BamHI (1381) XbaI * (1393) BclI * (1403) MfeI (1496) HpaI (1509) Bts α I (1585) MluI (1632) SexAI * (2335) pTagGFP2-C 4722 bp
PciI  (4664)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3844)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3735)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3262)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2979)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2864)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2864)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2848)
1 site
T G C G C A A C G C G T
MscI  (2828)
1 site
T G G C C A A C C G G T
PluTI  (2749)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2747)
1 site
G G C G C C C C G C G G
NarI  (2746)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2745)
1 site
G G C G C C C C G C G G
EagI  (2652)
1 site
C G G C C G G C C G G C
BspDI  (2586)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2586)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2567)
1 site
A G G C C T T C C G G A
SfiI  (2521)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
BmgBI  (669)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BstEII  (786)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
XmnI  (850)
1 site
G A A N N N N T T C C T T N N N N A A G
Bpu10I  (1207)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BspEI  (1321)
1 site
T C C G G A A G G C C T
BglII  (1330)
1 site
A G A T C T T C T A G A
PaeR7I  (1334)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1334)
1 site
C T C G A G G A G C T C
Eco53kI  (1339)
1 site
G A G C T C C T C G A G
SacI  (1341)
1 site
G A G C T C C T C G A G
HindIII  (1343)
1 site
A A G C T T T T C G A A
EcoRI  (1350)
1 site
G A A T T C C T T A A G
PstI  (1359)
1 site
C T G C A G G A C G T C
SalI  (1360)
1 site
G T C G A C C A G C T G
AccI  (1361)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1366)
1 site
G G T A C C C C A T G G
KpnI  (1370)
1 site
G G T A C C C C A T G G
PspOMI  (1374)
1 site
G G G C C C C C C G G G
TspMI  (1377)
1 site
C C C G G G G G G C C C
XmaI  (1377)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1378)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1379)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1381)
1 site
G G A