Resources
Plasmid Files

pTurboFP602-B

Vector for expressing TurboFP602 in bacteria.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pTurboFP602-B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
Download Free Trial Get SnapGene Viewer


AvaI - BsoBI - PaeR7I - XhoI (1) EcoO109I (4099) AatII (4045) ZraI (4043) XmnI (3722) PvuI (3493) FspI (3345) AseI (3295) BsrFI (3203) AhdI (3123) AlwNI (2646) AflIII - PciI (2230) BspQI - SapI (2114) BstAPI (2054) BmeT110I (2) PsiI (49) MfeI (59) BseRI (115) BsaBI * (126) BamHI (127) BclI * (155) BsrGI (182) Bsu36I (398) SfiI (608) BsgI (637) BfuAI - BspMI (659) DraIII (726) PshAI (772) HindIII (840) BlpI (852) NheI (960) BmtI (964) Bpu10I (985) BspEI (1208) XbaI (1817) PfoI (1874) PflFI - Tth111I (1977) BsaAI (1984) AccI (2002) BstZ17I (2003) NdeI (2053) pTurboFP602-B 4115 bp
AvaI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
EcoO109I  (4099)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
AatII  (4045)
1 site
G A C G T C C T G C A G
ZraI  (4043)
1 site
G A C G T C C T G C A G
XmnI  (3722)
1 site
G A A N N N N T T C C T T N N N N A A G
PvuI  (3493)
1 site
C G A T C G G C T A G C
FspI  (3345)
1 site
T G C G C A A C G C G T
AseI  (3295)
1 site
A T T A A T T A A T T A
BsrFI  (3203)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
AhdI  (3123)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2646)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2230)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2230)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BspQI  (2114)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (2114)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
BstAPI  (2054)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
BmeT110I  (2)
1 site
C Y C G R G G R G C Y C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
BseRI  (115)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
BsaBI  (126)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BamHI  (127)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
BclI  (155)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BsrGI  (182)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
Bsu36I  (398)
1 site
C C T N A G