pTurboGFP-B

Vector for expressing TurboGFP in bacteria.

Sequence Author: Evrogen

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No matches
BbsI (4089) AatII (4033) ZraI (4031) PvuI (3481) FspI (3333) AseI (3283) NmeAIII (3259) BglI (3231) BsaI (3172) AhdI (3111) AlwNI (2634) AflIII - PciI (2218) BstAPI (2042) PaeR7I - XhoI (1) PsiI (49) MfeI (59) lac operator ATG BamHI (127) KasI (277) NarI (278) SfoI (279) PluTI (281) NgoMIV (469) EagI - NaeI (471) FseI (473) PaqCI (581) PstI (707) PspOMI (714) ApaI - BanII (718) HindIII (828) NheI (948) BmtI (952) Bpu10I (973) PvuII (1100) BspEI (1196) PasI (1432) MscI (1467) XbaI (1805) PfoI (1862) PflFI - Tth111I (1965) AccI (1990) BstZ17I (1991) NdeI (2041) pTurboGFP-B 4103 bp
BbsI  (4089)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
AatII  (4033)
1 site
G A C G T C C T G C A G
ZraI  (4031)
1 site
G A C G T C C T G C A G
PvuI  (3481)
1 site
C G A T C G G C T A G C
FspI  (3333)
1 site
T G C G C A A C G C G T
AseI  (3283)
1 site
A T T A A T T A A T T A
NmeAIII  (3259)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BglI  (3231)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BsaI  (3172)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (3111)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2634)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AflIII  (2218)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (2218)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
BstAPI  (2042)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
PaeR7I  (1)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1)
1 site
C T C G A G G A G C T C
PsiI  (49)
1 site
T T A T A A A A T A T T
MfeI  (59)
1 site
C A A T T G G T T A A C
BamHI  (127)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
KasI  (277)
1 site
G G C G C C C C G C G G
NarI  (278)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (279)
1 site
G G C G C C C C G C G G
PluTI  (281)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
NgoMIV  (469)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
EagI  (471)
1 site
C G G C C G G C C G G C
NaeI  (471)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
FseI  (473)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
PaqCI  (581)
1 site
C A C C T G C ( N ) 4 G T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
Sticky ends from different PaqCI sites may not be compatible.
Cleavage can be improved with PaqCI Activator.
PstI  (707)
1 site
C T G C A G G A C G T C
PspOMI  (714)
1 site
G G G C C C C C C G G G
ApaI  (718)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (718)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
HindIII  (828)
1 site
A A G C T T T T C G A A
NheI  (948)
1 site
G C T A G C C G A T C G
BmtI  (952)
1 site
G C T A G C C G A T C G
Bpu10I  (973)
1 site
C C T N A G C G G A N T C G

Cleavage may be enhanced when more than one copy of the Bpu10I recognition sequence is present.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
PvuII  (1100)
1 site
C A G C T G G T C G A C
BspEI  (1196)
1 site
T C C G G A A G G C C T
PasI  (1432)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
MscI  (1467)
1 site
T G G C C A A C C G G T
XbaI  (1805)
1 site
T C T A G A A G A T C T
PfoI  (1862)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
PflFI  (1965)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1965)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
AccI  (1990)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BstZ17I  (1991)
1 site
G T A T A C C A T A T G
NdeI  (2041)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
AmpR
3038 .. 3898  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 2:  
   3038 .. 3829  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3038 .. 3898  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
   Segment 1:  signal sequence  
   3830 .. 3898  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
3038 .. 3898  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
ATG
115 .. 117  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
115 .. 117  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
TurboGFP
133 .. 828  =  696 bp
231 amino acids  =  25.6 kDa
Product: improved green fluorescent protein from Pontellina plumata
mammalian codon-optimized
TurboGFP
133 .. 828  =  696 bp
231 amino acids  =  25.6 kDa
Product: improved green fluorescent protein from Pontellina plumata
mammalian codon-optimized
CmR
987 .. 1646  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
987 .. 1646  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
2279 .. 2867  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
2279 .. 2867  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
AmpR promoter
3899 .. 4003  =  105 bp
AmpR promoter
3899 .. 4003  =  105 bp
lambda t0 terminator
849 .. 943  =  95 bp
transcription terminator from phage lambda
lambda t0 terminator
849 .. 943  =  95 bp
transcription terminator from phage lambda
rrnB T1 terminator
1711 .. 1797  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
rrnB T1 terminator
1711 .. 1797  =  87 bp
transcription terminator T1 from the E. coli rrnB gene
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 1:  
   10 .. 24  =  15 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 2:  -35  
   25 .. 30  =  6 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 3:  
   31 .. 47  =  17 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
   Segment 4:  -10  
   48 .. 54  =  7 bp
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
T5 promoter
10 .. 54  =  45 bp
4 segments
bacteriophage T5 promoter for E. coli RNA polymerase, with embedded lac operator
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
62 .. 78  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
30 .. 46  =  17 bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).
ORF:  115 .. 828  =  714 bp
ORF:  237 amino acids  =  26.1 kDa
ORF:  602 .. 835  =  234 bp
ORF:  77 amino acids  =  8.0 kDa
ORF:  987 .. 1646  =  660 bp
ORF:  219 amino acids  =  25.7 kDa
ORF:  3168 .. 3434  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  3038 .. 3898  =  861 bp
ORF:  286 amino acids  =  31.5 kDa
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