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Plasmid Files

pTurboRFP-C

Vector for fusing TurboRFP to the N-terminus of a partner protein.

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pTurboRFP-C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Evrogen
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PciI (4655) EcoO109I (3835) PfoI (3512) RsrII (3253) BsrDI (2970) PflFI - Tth111I (2855) FspI (2839) PluTI (2740) SfoI (2738) NarI (2737) KasI (2736) EagI (2643) BspDI * - ClaI * (2577) StuI (2558) BseRI (2555) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) AgeI (600) BsrGI (650) BbsI (908) BsgI (1105) PshAI (1240) ScaI (1273) BspEI (1312) BglII (1321) PaeR7I - XhoI (1325) Eco53kI (1330) SacI (1332) HindIII (1334) EcoRI (1341) PstI (1350) SalI (1351) AccI (1352) Acc65I (1357) KpnI (1361) SacII (1364) PspOMI (1365) TspMI - XmaI (1368) ApaI (1369) SmaI (1370) BamHI (1372) XbaI * (1384) MfeI (1487) HpaI (1500) MluI (1623) SexAI * (2326) pTurboRFP-C 4713 bp
PciI  (4655)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
EcoO109I  (3835)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3512)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3253)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2970)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2855)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2855)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2839)
1 site
T G C G C A A C G C G T
PluTI  (2740)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
SfoI  (2738)
1 site
G G C G C C C C G C G G
NarI  (2737)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI  (2736)
1 site
G G C G C C C C G C G G
EagI  (2643)
1 site
C G G C C G G C C G G C
BspDI  (2577)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2577)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2558)
1 site
A G G C C T T C C G G A
BseRI  (2555)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
AgeI  (600)
1 site
A C C G G T T G G C C A
BsrGI  (650)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BbsI  (908)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BsgI  (1105)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PshAI  (1240)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for long incubations.
ScaI  (1273)
1 site
A G T A C T T C A T G A
BspEI  (1312)
1 site
T C C G G A A G G C C T
BglII  (1321)
1 site
A G A T C T T C T A G A
PaeR7I  (1325)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1325)
1 site
C T C G A G G A G C T C
Eco53kI  (1330)
1 site
G A G C T C C T C G A G
SacI  (1332)
1 site
G A G C T C C T C G A G
HindIII  (1334)
1 site
A A G C T T T T C G A A
EcoRI  (1341)
1 site
G A A T T C C T T A A G
PstI  (1350)
1 site
C T G C A G G A C G T C
SalI  (1351)
1 site
G T C G A C C A G C T G
AccI  (1352)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
Acc65I  (1357)
1 site
G G T A C C C C A T G G
KpnI  (1361)
1 site
G G T A C C C C A T G G
SacII  (1364)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1365)
1 site
G G G C C C C C C G G G
TspMI  (1368)
1 site
C C C G G G G G G C C C
XmaI  (1368)
1 site
C C<