Resources
Plasmid Files

pZsYellow1-C1

Vector for fusing ZsYellow1 to the N-terminus of a partner protein.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pZsYellow1-C1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
Download Free Trial Get SnapGene Viewer


PciI (4649) ApaLI (4335) EcoO109I (3829) BsaI (3720) PfoI (3506) RsrII (3247) BsrDI (2964) PflFI - Tth111I (2849) FspI (2833) MscI (2813) BspDI * - ClaI * (2571) StuI (2552) SfiI (2506) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) SgrAI (700) PflMI (1043) BmgBI (1104) BsrGI (1163) BstXI (1299) BglII (1315) PaeR7I - XhoI (1319) Eco53kI (1324) SacI (1326) HindIII (1328) EcoRI (1335) PstI (1344) SalI (1345) AccI (1346) SacII (1358) PspOMI (1359) TspMI - XmaI (1362) ApaI (1363) SmaI (1364) BamHI (1366) XbaI * (1378) MfeI (1481) HpaI (1494) Bts α I (1570) MluI (1617) DraIII (1847) SexAI * (2320) pZsYellow1-C1 4707 bp
PciI  (4649)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4335)
1 site
G T G C A C C A C G T G
EcoO109I  (3829)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3720)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
PfoI  (3506)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (3247)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (2964)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (2849)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (2849)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2833)
1 site
T G C G C A A C G C G T
MscI  (2813)
1 site
T G G C C A A C C G G T
BspDI  (2571)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2571)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
StuI  (2552)
1 site
A G G C C T T C C G G A
SfiI  (2506)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
AseI  (7)
1 site
A T T A A T T A A T T A
NdeI  (234)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (340)
1 site
T A C G T A A T G C A T
NheI  (591)
1 site
G C T A G C C G A T C G
BmtI  (595)
1 site
G C T A G C C G A T C G
AfeI  (596)
1 site
A G C G C T T C G C G A
SgrAI  (700)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
PflMI  (1043)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
BmgBI  (1104)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BsrGI  (1163)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BstXI  (1299)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BglII  (1315)
1 site
A G A T C T T C T A G A
PaeR7I  (1319)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1319)
1 site
C T C G A G G A G C T C
Eco53kI  (1324)
1 site
G A G C T C C T C G A G
SacI  (1326)
1 site
G A G C T C C T C G A G
HindIII  (1328)
1 site
A A G C T T T T C G A A
EcoRI  (1335)
1 site
G A A T T C C T T A A G
PstI  (1344)
1 site
C T G C A G G A C G T C
SalI  (1345)
1 site
G T C G A C C A G C T G
AccI  (1346)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
SacII  (1358)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
PspOMI  (1359)
1 site
G G G C C C C C C G G G
TspMI  (1362)
1 site
C C C G G G G G G C C C
XmaI  (1362)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
ApaI  (1363)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SmaI  (1364)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (1366)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (1378)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
MfeI  (1481)
1 site
C A A T T G G T T A A C
HpaI  (1494)
1 site
G T T A A C C A A T T G
BtsαI  (1570)
1 site
G C A G T G N N C G T C A C

Sticky ends from different BtsαI sites may not be compatible.
MluI  (1617)
1 site
A C G C G T T G C G C A
DraIII  (1847)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SexAI  (2320)
1 site
A C C W G G T