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Plasmid Files

pd2ECFP-N1

Vector for fusing destabilized ECFP, with an in vivo half-life of ~2 hours, to the C-terminus of a partner protein.

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pd2ECFP-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4804) ApaLI (4490) EcoO109I (3984) BsaI (3875) RsrII (3402) BsrDI (3119) PflFI - Tth111I (3004) FspI (2988) MscI (2968) PluTI (2889) SfoI (2887) NarI (2886) KasI (2885) BspDI * - ClaI * (2726) StuI (2707) SfiI (2661) SexAI * (2475) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) BsrGI (1388) BlpI (1399) NotI (1530) XbaI * (1540) MfeI (1636) HpaI (1649) AflII (1768) DraIII (2002) pd2ECFP-N1 4862 bp
AflIII  (4804)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4804)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4490)
1 site
G T G C A C C A C G T G
EcoO109I  (3984)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3875)
1 site
G G T C T C N C C A