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Plasmid Files

pd2EYFP-N1

Vector for fusing destabilized EYFP, with an in vivo half-life of ~2 hours, to the C-terminus of a partner protein.

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pd2EYFP-N1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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AflIII - PciI (4804) ApaLI (4490) EcoO109I (3984) BsaI (3875) RsrII (3402) BsrDI (3119) PflFI - Tth111I (3004) FspI (2988) MscI (2968) PluTI (2889) SfoI (2887) NarI (2886) KasI (2885) BspDI * - ClaI * (2726) StuI (2707) SfiI (2661) SexAI * (2475) AseI (7) NdeI (234) SnaBI (340) NheI (591) BmtI (595) AfeI (596) BglII (609) PaeR7I - XhoI (613) Eco53kI (618) SacI (620) EcoRI (629) SalI (639) AccI (640) Acc65I (645) KpnI (649) SacII (652) PspOMI (653) TspMI - XmaI (656) ApaI (657) SmaI (658) BamHI (660) AgeI (666) BsrGI (1388) BlpI (1399) NotI (1530) XbaI * (1540) MfeI (1636) HpaI (1649) AflII (1768) DraIII (2002) pd2EYFP-N1 4862 bp
AflIII  (4804)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (4804)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
ApaLI  (4490)
1 site
G T G C A C C A C G T G
EcoO109I  (3984)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
BsaI  (3875)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
RsrII  (3402)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
BsrDI  (3119)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
PflFI  (3004)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (3004)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
FspI  (2988)
1 site
T G C G C A A C G