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Plasmid Files

phKikGR1-S1

Vector for expressing hKikGR1 in bacteria.

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phKikGR1-S1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  MBL International
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PfoI (3315) FauNDI - NdeI (3180) HindIII (2963) BbuI - PaeI - SphI (2961) PstI (2955) AdeI - DraIII (2845) BsgI (2843) BstXI (2818) AjiI - BmgBI - BtrI (2743) BsaAI - BstBAI - Ppu21I (2716) BbsI - BpiI - BpuAI - BstV2I (2684) BbvCI - Bpu10I (2300) AleI - OliI (2264) Bsp19I - BstDSI - BtgI - NcoI (2262) BamHI (2253) SmaI (2250) Cfr9I - KpnI - TspMI - XmaI - XmaCI (2248) Acc65I - Asp718I (2244) Psp124BI - SacI - SstI (2242) Ecl136II - Eco53kI - EcoICRI (2240) AcsI - ApoI - EcoRI - XapI (2232) BspQI - LguI - PciSI - SapI (1993) AflIII - BspLU11I - PciI - PscI (1876) DraII - EcoO109I (8) ZraI (67) AatII (69) SspI (183) Asp700I - MroXI - PdmI - XmnI (388) Acc113I - BmcAI - ScaI - ZrmI (507) TsoI (590) NmeAIII (841) BsaI - Bso31I - BspTNI - Eco31I (922) AhdI - AspEI - BmeRI - DriI - Eam1105I (988) phKikGR1-S1 3366 bp
PfoI  (3315)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
FauNDI  (3180)
1 site
C A T A T G G T A T A C
NdeI  (3180)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
HindIII  (2963)
1 site
A A G C T T T T C G A A
BbuI  (2961)
1 site
G C A T G C C G T A C G
PaeI  (2961)
1 site
G C A T G C C G T A C G
SphI  (2961)
1 site
G C A T G C C G T A C G
PstI  (2955)
1 site
C T G C A G G A C G T C
AdeI  (2845)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different AdeI sites may not be compatible.
DraIII  (2845)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsgI  (2843)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BstXI  (2818)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AjiI  (2743)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by AjiI will not always regenerate an AjiI site.
BmgBI  (2743)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BtrI  (2743)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BtrI will not always regenerate a BtrI site.
BsaAI  (2716)
1 site
Y A C G T R R T G C A Y
BstBAI  (2716)
1 site
Y A C G T R R T G C A Y
Ppu21I  (2716)
1 site
Y A C G T R R T G C A Y
BbsI  (2684)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BpiI  (2684)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpiI sites may not be compatible.
BpuAI  (2684)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BpuAI sites may not be compatible.
BstV2I  (2684)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BstV2I sites may not be compatible.
BbvCI  (2300)
1 site
C C T C A G C G G A G T C G
Bpu10I  (2300)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
AleI  (2264)
1 site
C A C N N N N G T G G T G N N N N C A C
OliI  (2264)
1 site
C A C N N N N G T G G T G N N N N C A C
Bsp19I  (2262)
1 site
C C A T G G G G T A C C
BstDSI  (2262)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BstDSI sites may not be compatible.
BtgI  (2262)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (2262)
1 site
C C A T G G G G T A C C
BamHI  (2253)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
SmaI  (2250)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Cfr9I  (2248)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the Cfr9I recognition sequence.
KpnI  (2248)
1 site
G G T A C C C C A T G G
TspMI  (2248)
1 site
C C C G G G G G G C C C
XmaI  (2248)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
XmaCI  (2248)
1 site
C C C G G G G G G C C C
Acc65I  (2244)
1 site
G G T A C C C C A T G G
Asp718I  (2244)
1 site
G G T A C C C C A T G G
Psp124BI  (2242)
1 site
G A G C T C C T C G A G
SacI  (2242)
1 site
G A G C T C C T C G A G
SstI  (2242)
1 site
G A G C T C C T C G A G
Ecl136II  (2240)
1 site
G A G C T C C T C G A G
Eco53kI  (2240)
1 site
G A G C T C C T C G A G
EcoICRI  (2240)
1 site
G A G C T C C T C G A G
AcsI  (2232)
1 site
R A A T T Y Y T T A A R
ApoI  (2232)
1 site
R A A T T Y Y T T