Resources
Plasmid Files

phMGFP

Vector encoding the Monster Green® fluorescent protein (Monster GFP).

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

phMGFP Sequence and MapphMGFP.dna
Map and Sequence File   
Sequence Author:  Promega
Download Free Trial Get SnapGene Viewer

 BglII (4702) PspFI (4390) BseYI (4386) AlwNI (4281) AhdI (3802) BpmI (3733) NmeAIII (3655) XmnI (3202) EcoO109I (2822) NspI (2777) PfoI (2763) SpeI (152) SnaBI (493) Eco53kI (719) SacI (721) HindIII (748) PstI (830) BfuAI - BspMI (844) NheI (1052) BmtI (1056) TspMI - XmaI (1058) SmaI (1060) EcoRV (1066) BclI * (1083) AleI (1250) BstEII (1265) PflFI - Tth111I (1448) BstXI (1627) BsgI (1652) PflMI (1654) NgoMIV (1751) NaeI (1753) XbaI (1764) EagI - NotI (1771) HpaI (1937) MfeI (1946) BsaBI * (2039) BspDI * - ClaI * (2043) phMGFP 4707 bp
BglII  (4702)
1 site
A G A T C T T C T A G A
PspFI  (4390)
1 site
C C C A G C G G G T C G
BseYI  (4386)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
AlwNI  (4281)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (3802)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BpmI  (3733)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (3655)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
XmnI  (3202)
1 site
G A A N N N N T T C C T T N N N N A A G
EcoO109I  (2822)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
NspI  (2777)
1 site
R C A T G Y Y G T A C R
PfoI  (2763)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
SpeI  (152)
1 site
A C T A G T T G A T C A
SnaBI  (493)
1 site
T A C G T A A T G C A T
Eco53kI  (719)
1 site
G A G C T C C T C G A G
SacI  (721)
1 site
G A G C T C C T C G A G
HindIII  (748)
1 site
A A G C T T T T C G A A
PstI  (830)
1 site
C T G C A G G A C G T C
BfuAI  (844)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (844)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
NheI  (1052)
1 site
G C T A G C C G A T C G
BmtI  (1056)
1 site
G C T A G C C G A T C G
TspMI  (1058)
1 site
C C C G G G G G G C C C
XmaI  (1058)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1060)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRV  (1066)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BclI  (1083)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AleI  (1250)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (1265)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PflFI  (1448)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1448)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BstXI  (1627)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BsgI  (1652)
1 site
G T G C A G ( N ) 14 N N C A C G T C ( N ) 14

Efficient cleavage requires at least two copies of the BsgI
recognition sequence.
Sticky ends from different BsgI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PflMI  (1654)
1 site
C C A N N N N N T G G G G T N N N N N A C C

Sticky ends from different PflMI sites may not be compatible.
NgoMIV  (1751)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
NaeI  (1753)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
XbaI  (1764)
1 site
T C T A G A A G A T C T
EagI  (1771)
1 site
C G G C C G G C C G G C
NotI  (1771)
1 site
G C G G C C G C C G C C G G C G
HpaI  (1937)
1 site
G T T A A C C A A T T G
MfeI  (1946)
1 site
C A A T T G G T T A A C
BsaBI  (2039)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BspDI  (2043)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
ClaI  (2043)
1 site
A T C G A T T A G C T A
* Blocked by Dam methylation.
AmpR
3015 .. 3875  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   3015 .. 3083  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3015 .. 3875  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   3084 .. 3875  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
3015 .. 3875  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
hMGFP
1076 .. 1759  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein
(Monster GFP), a modified version of the GFP from
Montastrea cavernosa
mammalian codon-optimized with few consensus
transcription factor binding sites
hMGFP
1076 .. 1759  =  684 bp
227 amino acids  =  25.9 kDa
Product: Monster Green® fluorescent protein
(Monster GFP), a modified version of the GFP from
Montastrea cavernosa
mammalian codon-optimized with few consensus
transcription factor binding sites
ori
4046 .. 4634  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
4046 .. 4634  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
2123 .. 2578  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
2123 .. 2578  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
214 .. 517  =  304 bp
human cytomegalovirus immediate early enhancer
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
518 .. 721  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and
immunoglobulin heavy chain genes
chimeric intron
857 .. 989  =  133 bp
chimera between introns from human β-globin and
immunoglobulin heavy chain genes
SV40 poly(A) signal
1816 .. 1937  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
1816 .. 1937  =  122 bp
SV40 polyadenylation signal
AmpR promoter
2910 .. 3014  =  105 bp
AmpR promoter
2910 .. 3014  =  105 bp
T7 promoter
1034 .. 1052  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
1034 .. 1052  =  19 bp
promoter for bacteriophage T7 RNA polymerase
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter