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Plasmid Files

phmUkG1-S1

Vector for expressing hmUkG1 in bacteria.

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phmUkG1-S1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  MBL International
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PfoI (3312) BstAPI (3181) FauNDI - NdeI (3177) HindIII (2960) BbuI - PaeI - SphI (2958) PstI (2952) Bpu10I (2858) BstXI (2809) AjiI - BmgBI - BtrI (2734) BsaAI - BstBAI - Ppu21I (2707) BbsI - BpiI - BpuAI - BstV2I (2675) BseRI (2297) BsaBI * - Bse8I - BseJI * - MamI * (2291) BclI * - FbaI * - Ksp22I * (2274) AleI - OliI (2264) Bsp19I - BssT1I - Eco130I - EcoT14I - ErhI - NcoI - StyI (2262) BamHI (2253) SmaI (2250) Cfr9I - KpnI - TspMI - XmaI - XmaCI (2248) Acc65I - Asp718I (2244) Psp124BI - SacI - SstI (2242) Ecl136II - Eco53kI - EcoICRI (2240) AcsI - ApoI - EcoRI - XapI (2232) BspQI - LguI - PciSI - SapI (1993) AflIII - BspLU11I - PciI - PscI (1876) DraII - EcoO109I (8) ZraI (67) AatII (69) SspI (183) Asp700I - MroXI - PdmI - XmnI (388) Acc113I - BmcAI - ScaI - ZrmI (507) TsoI (590) NmeAIII (841) Bse118I - BsrFI - BssAI - Cfr10I (903) BsaI - Bso31I - BspTNI - Eco31I (922) AhdI - AspEI - BmeRI - DriI - Eam1105I (988) phmUkG1-S1 3363 bp
PfoI  (3312)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
BstAPI  (3181)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
FauNDI  (3177)
1 site
C A T A T G G T A T A C
NdeI  (3177)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
HindIII  (2960)
1 site
A A G C T T T T C G A A
BbuI  (2958)
1 site
G C A T G C C G T A C G
PaeI  (2958)
1 site
G C A T G C C G T A C G
SphI  (2958)
1 site
G C A T G C C G T A C G
PstI  (2952)
1 site
C T G C A G G A C G T C
Bpu10I  (2858)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstXI  (2809)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AjiI  (2734)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by AjiI will not always regenerate an AjiI site.
BmgBI  (2734)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
BtrI  (2734)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BtrI will not always regenerate a BtrI site.