Plasmid Files

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AflIII  (5385) 1 site

Sticky ends from different AflIII sites may not be compatible.

PciI  (5385) 1 site

PciI is inhibited by nonionic detergents.

ApaLI  (5071) 1 site

BsaI  (4456) 1 site

Sticky ends from different BsaI sites may not be compatible. BsaI can be used between 37°C and 50°C.

PfoI  (4242) 1 site

Sticky ends from different PfoI sites may not be compatible.

RsrII  (3983) 1 site

Efficient cleavage requires at least two copies of the RsrII recognition sequence. Sticky ends from different RsrII sites may not be compatible. For full activity, add fresh DTT.

BsrDI  (3700) 1 site

Sticky ends from different BsrDI sites may not be compatible.

PflFI  (3585) 1 site

The 1-base overhangs produced by PflFI may be hard to ligate. Sticky ends from different PflFI sites may not be compatible.

Tth111I  (3585) 1 site

The 1-base overhangs produced by Tth111I may be hard to ligate. Sticky ends from different Tth111I sites may not be compatible.

PluTI  (3470) 1 site

Efficient cleavage requires at least two copies of the PluTI recognition sequence.

SfoI  (3468) 1 site

NarI  (3467) 1 site

Efficient cleavage requires at least two copies of the NarI recognition sequence.

KasI  (3466) 1 site

BspDI  (3307) 1 site

* Blocked by Dam methylation.

ClaI  (3307) 1 site

* Blocked by Dam methylation.

SfiI  (3242) 1 site

Efficient cleavage requires at least two copies of the SfiI recognition sequence. Sticky ends from different SfiI sites may not be compatible.

AseI  (7) 1 site

NdeI  (234) 1 site

Prolonged incubation with NdeI may lead to removal of additional nucleotides.

SnaBI  (340) 1 site

NheI  (591) 1 site

BmtI  (595) 1 site

BglII  (609) 1 site

PaeR7I  (613) 1 site

PaeR7I does not recognize the sequence CTCTCGAG.

XhoI  (613) 1 site

Eco53kI  (618) 1 site

SacI  (620) 1 site

HindIII  (622) 1 site

EcoRI  (629) 1 site

SalI  (639) 1 site

AccI  (640) 1 site

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence. Sticky ends from different AccI sites may not be compatible.

Acc65I  (645) 1 site

KpnI  (649) 1 site

SacII  (652) 1 site

Efficient cleavage requires at least two copies of the SacII recognition sequence.

PspOMI  (653) 1 site

TspMI  (656) 1 site

XmaI  (656) 1 site

Efficient cleavage requires at least two copies of the XmaI recognition sequence. Full cleavage with XmaI may require a long incubation.

ApaI  (657) 1 site

ApaI can be used between 25°C and 37°C.

SmaI  (658) 1 site

SmaI can be used at 37°C for brief incubations.

BamHI  (660) 1 site

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.

AgeI  (666) 1 site

AgeI quickly loses activity at 37°C, but can be used at 25°C for long incubations.

BsrGI  (2099) 1 site

BsrGI is typically used at 37°C, but is even more active at 60°C.

NotI  (2111) 1 site

XbaI  (2121) 1 site

* Blocked by Dam methylation.

MfeI  (2217) 1 site

HpaI  (2230) 1 site

BtsαI  (2306) 1 site

Sticky ends from different BtsI sites may not be compatible.

AflII  (2349) 1 site

The sticky ends produced by AflII are hard to ligate.

DraIII  (2583) 1 site

Sticky ends from different DraIII sites may not be compatible.

tdTomato 679 .. 2109  =  1431 bp 476 amino acids  =  54.2 kDa Product: tandem dimeric (pseudo-monomeric) derivative of DsRed mammalian codon-optimized

tdTomato 679 .. 2109  =  1431 bp 476 amino acids  =  54.2 kDa Product: tandem dimeric (pseudo-monomeric) derivative of DsRed mammalian codon-optimized

NeoR/KanR 3339 .. 4133  =  795 bp 264 amino acids  =  29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin)

NeoR/KanR 3339 .. 4133  =  795 bp 264 amino acids  =  29.0 kDa Product: aminoglycoside phosphotransferase from Tn5 confers resistance to neomycin, kanamycin, and G418 (Geneticin)

ori 4741 .. 5329  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori 4741 .. 5329  =  589 bp high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori 2359 .. 2814  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori 2359 .. 2814  =  456 bp f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

SV40 promoter 2947 .. 3304  =  358 bp SV40 enhancer and early promoter

SV40 promoter 2947 .. 3304  =  358 bp SV40 enhancer and early promoter

CMV enhancer 61 .. 364  =  304 bp human cytomegalovirus immediate early enhancer

CMV enhancer 61 .. 364  =  304 bp human cytomegalovirus immediate early enhancer

CMV promoter 365 .. 568  =  204 bp human cytomegalovirus (CMV) immediate early promoter

CMV promoter 365 .. 568  =  204 bp human cytomegalovirus (CMV) immediate early promoter

SV40 poly(A) signal 2231 .. 2352  =  122 bp SV40 polyadenylation signal

SV40 poly(A) signal 2231 .. 2352  =  122 bp SV40 polyadenylation signal

AmpR promoter 2841 .. 2945  =  105 bp

AmpR promoter 2841 .. 2945  =  105 bp

MCS 591 .. 671  =  81 bp multiple cloning site of fluorescent protein plasmids

MCS 591 .. 671  =  81 bp multiple cloning site of fluorescent protein plasmids

HSV TK poly(A) signal 4365 .. 4412  =  48 bp herpesvirus thymidine kinase polyadenylation signal

HSV TK poly(A) signal 4365 .. 4412  =  48 bp herpesvirus thymidine kinase polyadenylation signal

SV40 ori 3155 .. 3290  =  136 bp SV40 origin of replication

SV40 ori 3155 .. 3290  =  136 bp SV40 origin of replication

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