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Plasmid Files

rsEGFP2

Reversibly and rapidly switchable enhanced green fluorescent protein.

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rsEGFP2 Sequence and MaprsEGFP2.dna
Map and Sequence File   
Sequence Author:  Hell/Jakobs Lab
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 600 400 200 rsEGFP2 End (717) BsrGI (710) TaqII (625) BlpI (622) BsiHKAI (610) BmrI (606) Bpu10I (604) BstYI (500) PfoI * (281) BssS α I (181) BsrFI (151) BtgZI (122) BanI (36) BseRI (31) Start (0) rsEGFP2 717 bp
End  (717)
0 sites
BsrGI  (710)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TaqII  (625)
1 site
G A C C G A ( N ) 9 N N C T G G C T ( N ) 9

Sticky ends from different TaqII sites may not be compatible.
BlpI  (622)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BsiHKAI  (610)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
BmrI  (606)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
Bpu10I  (604)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BstYI  (500)
1 site
R G A T C Y Y C T A G R
PfoI  (281)
1 site
T C C N G G A A G G N C C T
* Blocked by Dcm methylation.
Sticky ends from different PfoI sites may not be compatible.
BssSαI  (181)
1 site
C A C G A G G T G C T C
BsrFI  (151)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI  (122)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BanI  (36)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BseRI  (31)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
Start  (0)
0 sites
rsEGFP2
1 .. 717  =  717 bp
239 amino acids  =  26.9 kDa
Product: reversibly and rapidly switchable enhanced
green fluorescent protein (Grotjohann et al., 2012)
mammalian codon-optimized
rsEGFP2
1 .. 717  =  717 bp
239 amino acids  =  26.9 kDa
Product: reversibly and rapidly switchable enhanced
green fluorescent protein (Grotjohann et al., 2012)
mammalian codon-optimized
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