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Plasmid Files

superfolder GFP

GFP variant that folds robustly even when fused to poorly folded proteins.

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superfolder GFP Sequence and Mapsuperfolder GFP.dna
Map and Sequence File   
Sequence Author:  Waldo Lab
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 600 400 200 superfolder GFP End (714) BanII - BsiHKAI - SacI (707) Eco53kI (705) PspFI (683) BpuEI (682) BseYI (679) DrdI (652) BstYI (627) BstBI (622) HincII (605) SalI (603) BtgZI (581) MfeI (560) AclI (509) BstZ17I (450) BmeT110I (422) AvaI - BsoBI - PaeR7I - PspXI - XhoI (421) DraI (390) AcuI (350) AflIII - MluI (322) EcoO109I - PpuMI (311) BsrGI (276) BsaWI - BspEI * (222) MscI (171) EaeI (169) NcoI - StyI (166) BaeGI - Bme1580I (74) BpmI (47) Start (0) superfolder GFP 714 bp
End  (714)
0 sites
BanII  (707)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BsiHKAI  (707)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
SacI  (707)
1 site
G A G C T C C T C G A G
Eco53kI  (705)
1 site
G A G C T C C T C G A G
PspFI  (683)
1 site
C C C A G C G G G T C G
BpuEI  (682)
1 site
C T T G A G ( N ) 14 N N G A A C T C ( N ) 14

Sticky ends from different BpuEI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BseYI  (679)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
DrdI  (652)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BstYI  (627)
1 site
R G A T C Y Y C T A G R
BstBI  (622)
1 site
T T C G A A A A G C T T
HincII  (605)
1 site
G T Y R A C C A R Y T G
SalI  (603)
1 site
G T C G A C C A G C T G
BtgZI  (581)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
MfeI  (560)
1 site
C A A T T G G T T A A C
AclI  (509)
1 site
A A C G T T T T G C A A
BstZ17I  (450)
1 site
G T A T A C C A T A T G
BmeT110I  (422)
1 site
C Y C G R G G R G C Y C
AvaI  (421)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (421)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures
up to 65°C.
PaeR7I  (421)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (421)
1 site
V C T C G A G B B G A G C T C V
XhoI  (421)
1 site
C T C G A G G A G C T C
DraI  (390)
1 site
T T T A A A A A A T T T
AcuI  (350)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AflIII  (322)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
MluI  (322)
1 site
A C G C G T T G C G C A
EcoO109I  (311)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (311)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
BsrGI  (276)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
BsaWI  (222)
1 site
W C C G G W W G G C C W

Efficient cleavage requires at least two copies of the BsaWI
recognition sequence.
BspEI  (222)
1 site
T C C G G A A G G C C T
* Blocked by Dam methylation.
MscI  (171)
1 site
T G G C C A A C C G G T
EaeI  (169)
1 site
Y G G C C R R C C G G Y
NcoI  (166)
1 site
C C A T G G G G T A C C
StyI  (166)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BaeGI  (74)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (74)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BpmI  (47)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
Start  (0)
0 sites
superfolder GFP
1 .. 714  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when
fused to poorly folded proteins (Pédelacq et al.,
2006)
superfolder GFP
1 .. 714  =  714 bp
238 amino acids  =  26.8 kDa
Product: GFP variant that folds robustly even when
fused to poorly folded proteins (Pédelacq et al.,
2006)
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