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Plasmid Files

pENTR™3C

Gateway® Dual Selection Vector for creating an entry vector by restriction cloning. Other reading frames are available with pENTR™1A and pENTR™2B.

To see this sequence with restriction sites, features, and translations, please download
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pENTR3C.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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NspI (3754) AflIII - PciI (3750) DrdI (3648) BssS α I (3577) BciVI (3552) HaeII (3510) AcuI (3208) AseI (2831) AsiSI - PvuI (2632) EcoNI (2544) NruI (2289) BspHI (2156) EcoRV (1978) PaeR7I - XhoI (1971) BfuAI - BspMI (1937) NheI (44) BmtI (48) ZraI (64) AatII (66) BbsI (134) BmrI (141) BsrBI (199) AflII (343) XmnI (470) Acc65I (490) KpnI (494) BspEI (819) BpmI (945) PasI (1055) BtgI - NcoI - StyI (1124) ScaI (1240) BssHII (1316) BstZ17I (1357) BbvCI (1547) TspMI - XmaI (1691) SmaI - SrfI (1693) BsaBI * (1706) BmgBI (1727) BstXI (1810) BsaI (1831) pENTR™3C 3756 bp
NspI  (3754)
1 site
R C A T G Y Y G T A C R
AflIII  (3750)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (3750)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
DrdI  (3648)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BssSαI  (3577)
1 site
C A C G A G G T G C T C
BciVI  (3552)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
HaeII  (3510)
1 site
R G C G C Y Y C G C G R
AcuI  (3208)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
AseI  (2831)
1 site
A T T A A T T A A T T A
AsiSI  (2632)
1 site
G C G A T C G C C G C T A G C G
PvuI  (2632)
1 site
C G A T C G G C T A G C
EcoNI  (2544)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
NruI  (2289)
1 site
T C G C G A A G C G C T
BspHI  (2156)
1 site
T C A T G A A G T A C T
EcoRV  (1978)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PaeR7I  (1971)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1971)
1 site
C T C G A G G A G C T C
BfuAI  (1937)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1937)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
NheI  (44)
1 site
G C T A G C C G A T C G
BmtI  (48)
1 site
G C T A G C C G A T C G
ZraI  (64)
1 site
G A C G T C C T G C A G
AatII  (66)
1 site
G A C G T C C T G C A G
BbsI  (134)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BmrI  (141)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BsrBI  (199)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C.
AflII  (343)
1 site
C T T A A G G A A T T C
XmnI  (470)
1 site
G A A N N N N T T C C T T N N N N A A G
Acc65I  (490)
1 site
G G T A C C C C A T G G
KpnI  (494)
1 site
G G T A C C C C A T G G
BspEI  (819)
1 site
T C C G G A A G G C C T
BpmI  (945)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
PasI  (1055)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BtgI  (1124)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (1124)
1 site
C C A T G G G G T A C C
StyI  (1124)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
ScaI  (1240)
1 site
A G T A C T T C A T G A
BssHII  (1316)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BstZ17I  (1357)
1 site
G T A T A C C A T A T G
BbvCI  (1547)
1 site
C C T C A G C G G A G T C G
TspMI  (1691)
1 site
C C C G G G G G G C C C
XmaI  (1691)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1693)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
SrfI  (1693)
1 site
G C C C G G G C C G G G C C C G
BsaBI  (1706)
1 site
G A T N N N N