Resources
Plasmid Files

pEXP2-DEST

Gateway® destination vector for cell-free expression and C-terminal tagging.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pEXP2-DEST Sequence and MappEXP2-DEST.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 BspQI - SapI (4340) NspI (4227) PciI (4223) DrdI (4121) PspFI (3924) BseYI (3920) AhdI (3338) BmrI (3298) FspI (3115) PvuI (2969) XmnI (2738) DraIII (2509) FseI (2432) SexAI * (2319) SgrAI (2270) AatII (2202) XbaI (58) NotI (224) PvuII (444) ApoI - EcoRI (544) PasI - PflMI * (776) SspI (856) BglII (991) BstZ17I (1079) BbvCI (1269) SrfI (1415) BstXI (1532) BfuAI - BspMI (1659) PstI (1670) SalI (1672) BstBI (1810) HindIII (1813) V5 tag MluI (1861) AgeI (1867) PmeI (1897) BlpI (1929) EcoO109I (1956) SfiI (2156) MauBI (2192) ZraI (2200) pEXP2-DEST 4403 bp
BspQI  (4340)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (4340)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
NspI  (4227)
1 site
R C A T G Y Y G T A C R
PciI  (4223)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
DrdI  (4121)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
PspFI  (3924)
1 site
C C C A G C G G G T C G
BseYI  (3920)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
AhdI  (3338)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmrI  (3298)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
FspI  (3115)
1 site
T G C G C A A C G C G T
PvuI  (2969)
1 site
C G A T C G G C T A G C
XmnI  (2738)
1 site
G A A N N N N T T C C T T N N N N A A G
DraIII  (2509)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
FseI  (2432)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SexAI  (2319)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
SgrAI  (2270)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI
recognition sequence.
AatII  (2202)
1 site
G A C G T C C T G C A G
XbaI  (58)
1 site
T C T A G A A G A T C T
NotI  (224)
1 site
G C G G C C G C C G C C G G C G
PvuII  (444)
1 site
C A G C T G G T C G A C
ApoI  (544)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (544)
1 site
G A A T T C C T T A A G
PasI  (776)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (776)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
SspI  (856)
1 site
A A T A T T T T A T A A
BglII  (991)
1 site
A G A T C T T C T A G A
BstZ17I  (1079)
1 site
G T A T A C C A T A T G
BbvCI  (1269)
1 site
C C T C A G C G G A G T C G
SrfI  (1415)
1 site
G C C C G G G C C G G G C C C G
BstXI  (1532)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
BfuAI  (1659)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1659)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
PstI  (1670)
1 site
C T G C A G G A C G T C
SalI  (1672)
1 site
G T C G A C C A G C T G
BstBI  (1810)
1 site
T T C G A A A A G C T T
HindIII  (1813)
1 site
A A G C T T T T C G A A
MluI  (1861)
1 site
A C G C G T T G C G C A
AgeI  (1867)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
PmeI  (1897)
1 site
G T T T A A A C C A A A T T T G
BlpI  (1929)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
EcoO109I  (1956)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
SfiI  (2156)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
MauBI  (2192)
1 site
C G C G C G C G G C G C G C G C
ZraI  (2200)
1 site
G A C G T C C T G C A G
AmpR
2551 .. 3411  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 1:  signal sequence  
   2551 .. 2619  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2551 .. 3411  =  861 bp
286 amino acids  =  31.5 kDa
   Segment 2:  
   2620 .. 3411  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
2551 .. 3411  =  861 bp
286 amino acids  =  31.5 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
331 .. 990  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
331 .. 990  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
3582 .. 4167  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3582 .. 4167  =  586 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
BleoR
2156 .. 2530  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
BleoR
2156 .. 2530  =  375 bp
124 amino acids  =  13.8 kDa
Product: antibiotic-binding protein
confers resistance to bleomycin, phleomycin, and
Zeocin™
ccdB
1332 .. 1637  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
1332 .. 1637  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
attR1
98 .. 222  =  125 bp
recombination site for the Gateway® LR reaction
attR1
98 .. 222  =  125 bp
recombination site for the Gateway® LR reaction
attR2
1678 .. 1802  =  125 bp
recombination site for the Gateway® LR reaction
attR2
1678 .. 1802  =  125 bp
recombination site for the Gateway® LR reaction
T7 terminator
1940 .. 1987  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
1940 .. 1987  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
V5 tag
1822 .. 1863  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
1822 .. 1863  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
lac UV5 promoter
247 .. 277  =  31 bp
   Segment 1:  -35  
   247 .. 252  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
247 .. 277  =  31 bp
   Segment 2:  
   253 .. 270  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
247 .. 277  =  31 bp
   Segment 3:  -10  
   271 .. 277  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
247 .. 277  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
T7 promoter
20 .. 38  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
20 .. 38  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
1873 .. 1890  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
1873 .. 1890  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter