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Plasmid Files

pHELLSGATE 8

High-throughput binary Gateway® vector for silencing genes in plants using intron-containing hairpin RNA.

 
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pHELLSGATE 8.dna
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AgeI (17,051) XbaI (16,707) XbaI (15,280) HindIII (15,268) BspDI - ClaI (15,263) PacI (15,152) KpnI (14,466) Acc65I (14,462) EcoRI (14,456) XhoI (14,450) PspXI - XhoI (13,020) ScaI (12,386) StuI (12,234) SacI (11,646) Eco53kI (11,644) PmeI (11,220) BsiWI (11,055) FseI (10,595) Bsu36I (330) RsrII (1119) AflII (1923) MauBI (2118) BspEI (3460) AleI (5445) SexAI * (5533) FspAI (6101) NruI (6598) pHELLSGATE 8 17,485 bp
AgeI  (17,051)
1 site
A C C G G T T G G C C A
XbaI  (16,707)
2 sites
T C T A G A A G A T C T
XbaI  (15,280)
2 sites
T C T A G A A G A T C T
HindIII  (15,268)
1 site
A A G C T T T T C G A A
BspDI  (15,263)
1 site
A T C G A T T A