Resources
Plasmid Files

pLenti6/capTEV™-CT-DEST

Lentiviral Gateway® destination vector for expression of C-terminally capTEV™-tagged proteins.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pLenti6 capTEV-CT-DEST Sequence and MappLenti6 capTEV-CT-DEST.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
Download Free Trial Get SnapGene Viewer

 PciI (8581) AhdI (7693) DraIII (6553) SfiI (6065) SV40 poly(A) signal KpnI (5444) Acc65I (5440) BlpI (5421) EM7 promoter PmlI (4957) BclI * (4948) SexAI * (4664) AgeI (4545) EcoNI (942) KflI (1706) BspDI - ClaI (1795) NdeI (1989) SnaBI (2095) SpeI (2406) BspEI (2882) PasI (3118) BstZ17I (3421) SrfI (3757) BmgBI (3791) BfuAI - BspMI (4001) SalI (4014) attR2 PaeR7I - PspXI - XhoI (4169) PspOMI (4181) ApaI (4185) SacII (4188) BstBI (4192) BstEII (4283) MluI (4539) pLenti6/capTEV™-CT-DEST 8991 bp
PciI  (8581)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AhdI  (7693)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
DraIII  (6553)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SfiI  (6065)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
KpnI  (5444)
1 site
G G T A C C C C A T G G
Acc65I  (5440)
1 site
G G T A C C C C A T G G
BlpI  (5421)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
PmlI  (4957)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
BclI  (4948)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SexAI  (4664)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
AgeI  (4545)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
EcoNI  (942)
1 site
C C T N N N N N A G G G G A N N N N N T C C

The 1-base overhangs produced by EcoNI may be hard to ligate.
Sticky ends from different EcoNI sites may not be compatible.
KflI  (1706)
1 site
G G G W C C C C C C W G G G

Sticky ends from different KflI sites may not be compatible.
BspDI  (1795)
1 site
A T C G A T T A G C T A
ClaI  (1795)
1 site
A T C G A T T A G C T A
NdeI  (1989)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional
nucleotides.
SnaBI  (2095)
1 site
T A C G T A A T G C A T
SpeI  (2406)
1 site
A C T A G T T G A T C A
BspEI  (2882)
1 site
T C C G G A A G G C C T
PasI  (3118)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
BstZ17I  (3421)
1 site
G T A T A C C A T A T G
SrfI  (3757)
1 site
G C C C G G G C C G G G C C C G
BmgBI  (3791)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BmgBI will not always regenerate a BmgBI site.
BfuAI  (4001)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (4001)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
SalI  (4014)
1 site
G T C G A C C A G C T G
PaeR7I  (4169)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (4169)
1 site
V C T C G A G B B G A G C T C V
XhoI  (4169)
1 site
C T C G A G G A G C T C
PspOMI  (4181)
1 site
G G G C C C C C C G G G
ApaI  (4185)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SacII  (4188)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BstBI  (4192)
1 site
T T C G A A A A G C T T
BstEII  (4283)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
MluI  (4539)
1 site
A C G C G T T G C G C A
AmpR
6906 .. 7766  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6906 .. 6974  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6906 .. 7766  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   6975 .. 7766  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
6906 .. 7766  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
CmR
2673 .. 3332  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2673 .. 3332  =  660 bp
219 amino acids  =  25.7 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
7937 .. 8525  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
7937 .. 8525  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
f1 ori
6320 .. 6775  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
f1 ori
6320 .. 6775  =  456 bp
f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis
BSD
5026 .. 5424  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
BSD
5026 .. 5424  =  399 bp
132 amino acids  =  13.7 kDa
Product: blasticidin S deaminase
confers resistance to blasticidin
SV40 promoter
4583 .. 4912  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
4583 .. 4912  =  330 bp
SV40 enhancer and early promoter
ccdB
3674 .. 3979  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
ccdB
3674 .. 3979  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA
gyrase
Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains.
CMV enhancer
1816 .. 2119  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1816 .. 2119  =  304 bp
human cytomegalovirus immediate early enhancer
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
   Segment 1:  6xHis  
   4200 .. 4217  =  18 bp
   6 amino acids  =  840.9 Da
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
   Segment 2:  
   4218 .. 4220  =  3 bp
   1 amino acid  =  75.1 Da
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
   Segment 3:  TEV site  
   4221 .. 4241  =  21 bp
   7 amino acids  =  870.0 Da
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
   Segment 4:  
   4242 .. 4247  =  6 bp
   2 amino acids  =  259.3 Da
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
   Segment 5:  TEV site  
   4248 .. 4268  =  21 bp
   7 amino acids  =  870.0 Da
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
   Segment 6:  BioEase™ tag  
   4269 .. 4484  =  216 bp
   72 amino acids  =  7.1 kDa
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
capTEV™
4200 .. 4484  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
Product: composite tag for in vivo biotinylation, TEV
protease cleavage, and 6xHis tagging
RRE
1075 .. 1308  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
RRE
1075 .. 1308  =  234 bp
The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to
the cytoplasm.
3' LTR (ΔU3)
5511 .. 5744  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from
HIV-1
3' LTR (ΔU3)
5511 .. 5744  =  234 bp
self-inactivating 3' long terminal repeat (LTR) from
HIV-1
RSV promoter
1 .. 229  =  229 bp
Rous Sarcoma Virus enhancer/promoter
RSV promoter
1 .. 229  =  229 bp
Rous Sarcoma Virus enhancer/promoter
CMV promoter
2120 .. 2323  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
CMV promoter
2120 .. 2323  =  204 bp
human cytomegalovirus (CMV) immediate early
promoter
5' LTR (truncated)
230 .. 410  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
5' LTR (truncated)
230 .. 410  =  181 bp
truncated 5' long terminal repeat (LTR) from HIV-1
HIV-1 Ψ
457 .. 582  =  126 bp
packaging signal of human immunodeficiency virus
type 1
HIV-1 Ψ
457 .. 582  =  126 bp
packaging signal of human immunodeficiency virus
type 1
attR1
2440 .. 2564  =  125 bp
recombination site for the Gateway® LR reaction
attR1
2440 .. 2564  =  125 bp
recombination site for the Gateway® LR reaction
attR2
4020 .. 4144  =  125 bp
recombination site for the Gateway® LR reaction
attR2
4020 .. 4144  =  125 bp
recombination site for the Gateway® LR reaction
SV40 poly(A) signal
5816 .. 5937  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5816 .. 5937  =  122 bp
SV40 polyadenylation signal
AmpR promoter
6801 .. 6905  =  105 bp
AmpR promoter
6801 .. 6905  =  105 bp
EM7 promoter
4960 .. 5007  =  48 bp
synthetic bacterial promoter
EM7 promoter
4960 .. 5007  =  48 bp
synthetic bacterial promoter
V5 tag
4500 .. 4541  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
4500 .. 4541  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
lac UV5 promoter
2589 .. 2619  =  31 bp
   Segment 1:  -35  
   2589 .. 2594  =  6 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2589 .. 2619  =  31 bp
   Segment 2:  
   2595 .. 2612  =  18 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2589 .. 2619  =  31 bp
   Segment 3:  -10  
   2613 .. 2619  =  7 bp
E. coli lac promoter with an "up" mutation
lac UV5 promoter
2589 .. 2619  =  31 bp
3 segments
E. coli lac promoter with an "up" mutation
SV40 ori
4763 .. 4898  =  136 bp
SV40 origin of replication
SV40 ori
4763 .. 4898  =  136 bp
SV40 origin of replication
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter