pcDNA3.2 capTEV-CT V5-DEST

Gateway® destination vector for mammalian cell expression of proteins C-terminally modified with V5 and 6xHis tags plus a cleavable biotinylation peptide.

Sequence Author: Thermo Fisher (Invitrogen)

|Download SnapGene Viewer
Explore Over 2.7k Plasmids: Gateway® Cloning Vectors | More Plasmid Sets
No matches
SgrDI (7972) PvuI (7421) PciI (6158) PfoI (5582) RsrII (5321) PflFI - Tth111I (4923) EagI (4711) BclI * (4646) AvrII (4595) StuI (4594) CsiI - SexAI * (4362) DraIII (4072) SpeI (213) CMV enhancer NdeI (448) SnaBI (554) Eco53kI (780) SacI (782) BmgBI (1615) SrfI (1649) BbvCI (1792) EcoRI (2518) BspEI (2522) XbaI * (3145) PspOMI (3151) ApaI (3155) SacII (3158) BstBI (3162) AleI (3299) BstEII (3301) PmeI (3527) pcDNA™3.2/capTEV-CT/V5-DEST 8010 bp
SgrDI  (7972)
1 site
C G T C G A C G G C A G C T G C
PvuI  (7421)
1 site
C G A T C G G C T A G C
PciI  (6158)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PfoI  (5582)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
RsrII  (5321)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PflFI  (4923)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (4923)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
EagI  (4711)
1 site
C G G C C G G C C G G C
BclI  (4646)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AvrII  (4595)
1 site
C C T A G G G G A T C C
StuI  (4594)
1 site
A G G C C T T C C G G A
CsiI  (4362)
1 site
A C C W G G T T G G W C C A

Sticky ends from different CsiI sites may not be compatible.
SexAI  (4362)
1 site
A C C W G G T T G G W C C A
* Blocked by Dcm methylation.
Sticky ends from different SexAI sites may not be compatible.
DraIII  (4072)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
SpeI  (213)
1 site
A C T A G T T G A T C A
NdeI  (448)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI  (554)
1 site
T A C G T A A T G C A T
Eco53kI  (780)
1 site
G A G C T C C T C G A G
SacI  (782)
1 site
G A G C T C C T C G A G
BmgBI  (1615)
1 site
C A C G T C G T G C A G

This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
SrfI  (1649)
1 site
G C C C G G G C C G G G C C C G
BbvCI  (1792)
1 site
C C T C A G C G G A G T C G
EcoRI  (2518)
1 site
G A A T T C C T T A A G
BspEI  (2522)
1 site
T C C G G A A G G C C T
XbaI  (3145)
1 site
T C T A G A A G A T C T
* Blocked by Dam methylation.
PspOMI  (3151)
1 site
G G G C C C C C C G G G
ApaI  (3155)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
SacII  (3158)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstBI  (3162)
1 site
T T C G A A A A G C T T
AleI  (3299)
1 site
C A C N N N N G T G G T G N N N N C A C
BstEII  (3301)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PmeI  (3527)
1 site
G T T T A A A C C A A A T T T G
AmpR
6978 .. 7838  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 2:  
   6978 .. 7769  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6978 .. 7838  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
   Segment 1:  signal sequence  
   7770 .. 7838  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
6978 .. 7838  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
NeoR/KanR
4677 .. 5471  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
NeoR/KanR
4677 .. 5471  =  795 bp
264 amino acids  =  29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin®)
CmR
2083 .. 2736  =  654 bp
218 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
CmR
2083 .. 2736  =  654 bp
218 amino acids  =  25.6 kDa
Product: chloramphenicol acetyltransferase
confers resistance to chloramphenicol
ori
6219 .. 6807  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
ori
6219 .. 6807  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
f1 ori
3839 .. 4267  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
f1 ori
3839 .. 4267  =  429 bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis
CMV enhancer
199 .. 578  =  380 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
199 .. 578  =  380 bp
human cytomegalovirus immediate early enhancer
V5 tag
3167 .. 3208  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
3167 .. 3208  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
   Segment 1:  6xHis  
   3218 .. 3235  =  18 bp
   6 amino acids  =  840.9 Da
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
   Segment 2:  
   3236 .. 3238  =  3 bp
   1 amino acid  =  75.1 Da
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
   Segment 3:  TEV site  
   3239 .. 3259  =  21 bp
   7 amino acids  =  869.9 Da
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
   Segment 4:  
   3260 .. 3265  =  6 bp
   2 amino acids  =  259.3 Da
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
   Segment 5:  TEV site  
   3266 .. 3286  =  21 bp
   7 amino acids  =  869.9 Da
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
   Segment 6:  BioEase™ tag  
   3287 .. 3502  =  216 bp
   72 amino acids  =  7.1 kDa
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
capTEV™
3218 .. 3502  =  285 bp
95 amino acids  =  10.0 kDa
6 segments
Product: composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging
SV40 promoter
4281 .. 4610  =  330 bp
SV40 enhancer and early promoter
SV40 promoter
4281 .. 4610  =  330 bp
SV40 enhancer and early promoter
ccdB
1428 .. 1733  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
ccdB
1428 .. 1733  =  306 bp
101 amino acids  =  11.7 kDa
Product: CcdB, a bacterial toxin that poisons DNA gyrase
Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains.
CMV promoter
579 .. 782  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
CMV promoter
579 .. 782  =  204 bp
human cytomegalovirus (CMV) immediate early promoter
attR1
875 .. 999  =  125 bp
recombination site for the Gateway® LR reaction
attR1
875 .. 999  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3017 .. 3141  =  125 bp
recombination site for the Gateway® LR reaction
attR2
3017 .. 3141  =  125 bp
recombination site for the Gateway® LR reaction
SV40 poly(A) signal
5647 .. 5768  =  122 bp
SV40 polyadenylation signal
SV40 poly(A) signal
5647 .. 5768  =  122 bp
SV40 polyadenylation signal
AmpR promoter
7839 .. 7943  =  105 bp
AmpR promoter
7839 .. 7943  =  105 bp
cat promoter
2737 .. 2839  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
cat promoter
2737 .. 2839  =  103 bp
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
HSV TK poly(A) signal
3589 .. 3637  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
HSV TK poly(A) signal
3589 .. 3637  =  49 bp
herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)
T7 promoter
827 .. 845  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
827 .. 845  =  19 bp
promoter for bacteriophage T7 RNA polymerase
SV40 ori
4461 .. 4596  =  136 bp
SV40 origin of replication
SV40 ori
4461 .. 4596  =  136 bp
SV40 origin of replication
ORF:  4849 .. 5235  =  387 bp
ORF:  128 amino acids  =  14.6 kDa
ORF:  7108 .. 7374  =  267 bp
ORF:  88 amino acids  =  9.2 kDa
ORF:  4677 .. 5471  =  795 bp
ORF:  264 amino acids  =  29.0 kDa
ORF:  2053 .. 2736  =  684 bp
ORF:  227 amino acids  =  26.8 kDa
ORF:  1428 .. 1733  =  306 bp
ORF:  101 amino acids  =  11.7 kDa
ORF:  4986 .. 5240  =  255 bp
ORF:  84 amino acids  =  9.6 kDa
ORF:  6978 .. 7838  =  861 bp
ORF:  286 amino acids  =  31.6 kDa
Click here to try SnapGene

Download pcDNA3.2 capTEV-CT V5-DEST.dna file

SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Individual Sequences & Maps

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.