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Plasmid Files

pAMP10

Bacterial vector for inducible expression of a cloned cDNA. For an MCS with a NotI site, use pAMP1.

To see this sequence with restriction sites, features, and translations, please download
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pAMP10.dna
Map and Sequence File:    Download    Open   
Sequence Author:  I.M.A.G.E. Consortium
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DraIII (3890) PsiI (3762) XmnI (3346) ScaI (3227) TatI (3225) BsaI (2808) AhdI (2747) AlwNI (2270) ZraI (193) AatII (195) SphI (201) MluI (203) BsiWI (206) SnaBI (210) HindIII (213) BamHI (219) XbaI (225) FseI (238) SalI (266) AccI (267) AvaI - BsoBI - TspMI - XmaI (271) BmeT110I (272) SmaI (273) EcoRI (276) BspEI (280) RsrII (283) AgeI (285) Acc65I (288) KpnI (292) PstI - SbfI (297) BfuAI - BspMI (300) KasI (565) NarI * (566) SfoI (567) PluTI (569) BsmBI (589) EcoRV (758) PspOMI (997) ApaI (1001) BstEII (1022) BclI * (1190) PflMI (1629) MauBI (1661) PciI (1854) pAMP10 4122 bp
DraIII  (3890)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
PsiI  (3762)
1 site
T T A T A A A A T A T T
XmnI  (3346)
1 site
G A A N N N N T T C C T T N N N N A A G
ScaI  (3227)
1 site
A G T A C T T C A T G A
TatI  (3225)
1 site
W G T A C W W C A T G W
BsaI  (2808)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
AhdI  (2747)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
AlwNI  (2270)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ZraI  (193)
1 site
G A C G T C C T G C A G
AatII  (195)
1 site
G A C G T C C T G C A G
SphI  (201)
1 site
G C A T G C C G T A C G
MluI  (203)
1 site
A C G C G T T G C G C A
BsiWI  (206)
1 site
C G T A C G G C A T G C

BsiWI is typically used at 55°C, but is 50% active at 37°C.
SnaBI  (210)
1 site
T A C G T A A T G C A T
HindIII  (213)
1 site
A A G C T T T T C G A A
BamHI  (219)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
XbaI  (225)
1 site
T C T A G A A G A T C T
FseI  (238)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
SalI  (266)
1 site
G T C G A C C A G C T G
AccI  (267)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.
Sticky ends from different AccI sites may not be compatible.
AvaI  (271)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (271)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
TspMI  (271)
1 site
C C C G G G G G G C C C
XmaI  (271)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BmeT110I  (272)
1 site
C Y C G R G G R G C Y C
SmaI  (273)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EcoRI  (276)
1 site
G A A T T C C T T A A G
BspEI  (280)
1 site
T C C G G A A G G C C T
RsrII  (283)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
AgeI  (285)
1 site
A C C G G T T G G C C A
Acc65I  (288)
1 site
G G T A C C C C A T G G
KpnI  (292)
1 site
G G T A C C C C A T G G
PstI  (297)
1 site
C T G C A G G A C G T C
SbfI  (297)
1 site
C C T G C A G G G G A C G T C C
BfuAI  (300)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (300)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
KasI  (565)
1 site
G G C G C C C C G C G G
NarI  (566)
1 site
G G C G C C C C G C G G
* Blocked by Dcm methylation.
Efficient cleavage requires at least two copies of the NarI recognition sequence.
SfoI  (567)
1 site
G G C G C C C C G C G G
PluTI  (569)
1 site
G G C G C C C C G C G G

Efficient cleavage requires at least two copies of the PluTI recognition sequence.
BsmBI  (589)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
EcoRV  (758)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
PspOMI  (997)
1 site
G G G C C C C C C G G