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Plasmid Files

pDNR-Dual

Donor vector for transferring a cloned gene into an acceptor vector, and for tagging the gene, using Cre recombinase in the Creator™ system.

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pDNR-Dual.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BmtI (4888) NheI (4884) BglII (4856) MluI (4833) PspFI (4478) BseYI (4474) AlwNI (4369) AhdI (3890) BmrI (3850) BsaI (3824) BsrFI (3805) BglI (3772) NmeAIII (3743) AseI (3715) FspI (3667) PvuI (3521) XmnI (3290) SacII (2588) SalI (45) NdeI (61) TspMI - XmaI (66) SmaI (68) EcoRI (71) PstI - SbfI (81) BamHI (82) PaeR7I - XhoI (89) HindIII (95) XbaI (101) BssHII (118) PspOMI * (124) ApaI * (128) AvrII (180) SpeI (200) Eco53kI (314) SacI (316) NcoI (467) PasI (537) PflMI * (543) BsmBI (544) BspEI (772) BfuAI - BspMI (1079) BstZ17I (1380) StuI * (1750) BsgI (2074) PsiI (2149) BsrGI (2189) BsaAI - SnaBI (2258) pDNR-Dual 4938 bp
BmtI  (4888)
1 site
G C T A G C C G A T C G
NheI  (4884)
1 site
G C T A G C C G A T C G
BglII  (4856)
1 site
A G A T C T T C T A G A
MluI  (4833)
1 site
A C G C G T T G C G C A
PspFI  (4478)
1 site
C C C A G C G G G T C G
BseYI  (4474)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (4369)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (3890)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BmrI  (3850)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BsaI  (3824)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BsrFI  (3805)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its electrophoretic mobility.
BglI  (3772)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
NmeAIII  (3743)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
AseI  (3715)
1 site
A T T A A T T A A T T A
FspI  (3667)
1 site
T G C G C A A C G C G T
PvuI  (3521)
1 site
C G A T C G G C T A G C
XmnI  (3290)
1 site
G A A N N N N T T C C T T N N N N A A G
SacII  (2588)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
SalI  (45)
1 site
G T C G A C C A G C T G
NdeI  (61)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
TspMI  (66)
1 site
C C C G G G G G G C C C
XmaI  (66)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (68)
1 site
C C C G G G G G G C