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Plasmid Files

pOTB7-3

cDNA cloning vector with an f1 origin derived from pOTB7.

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pOTB7-3.dna
Map and Sequence File:    Download    Open   
Sequence Author:  I.M.A.G.E. Consortium
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SalI - SgrDI (2812) ScaI (2775) BtgI - NcoI - StyI (2659) MscI (2625) PasI - PflMI * (2590) BsmBI (2582) BpmI (2480) AclI (2445) BsrDI (2377) BspEI (2354) Bpu10I (2131) BspDI - ClaI (2080) BciVI (1862) BaeGI - Bme1580I (1750) ApaLI (1746) AlwNI (1651) AcuI (1518) AccI (2813) Bsu36I (141) StuI * (154) BglI (160) XhoI (185) BstXI (204) NspI (216) BspQI - EarI - SapI (329) NsiI (610) HindIII (613) Acc65I (619) KpnI (623) Eco53kI (627) SacI (629) BstXI (658) EcoRV (674) BsaBI (676) BstXI (684) XhoI (695) PI-SceI (717) BglII (788) AscI - BssHII (797) BsaI (819) HpaI (855) BfuAI - BspMI (866) AanI - AanI - AanI - PsiI (986) BsaAI - DraIII (1114) NgoMIV (1215) NaeI (1217) pOTB7-3 2816 bp
SalI  (2812)
1 site
G T C G A C C A G C T G
SgrDI  (2812)
1 site
C G T C G A C G G C A G C T G C
ScaI  (2775)
1 site
A G T A C T T C A T G A
BtgI  (2659)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
NcoI  (2659)
1 site
C C A T G G G G T A C C
StyI  (2659)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
MscI  (2625)
1 site
T G G C C A A C C G G T
PasI  (2590)
1 site
C C C W G G G G G G W C C C

Sticky ends from different PasI sites may not be compatible.
PflMI  (2590)
1 site
C C A N N N N N T G G G G T N N N N N A C C
* Blocked by Dcm methylation.
Sticky ends from different PflMI sites may not be compatible.
BsmBI  (2582)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BpmI  (2480)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AclI  (2445)
1 site
A A C G T