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Plasmid Files

pYX-Asc

Compact cDNA cloning vector.

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pYX-Asc Sequence and MappYX-Asc.dna
Map and Sequence File   
Sequence Author:  I.M.A.G.E. Consortium
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 SspI (0) EarI (1682) XmnI (1486) DraI (1464) BsaHI (1424) ScaI (1367) TatI (1365) TsoI (1286) PvuI (1257) FspI (1109) NmeAIII (1035) BsrFI (967) BpmI (957) BsaI (948) BmrI (927) AhdI (887) PacI (6) EcoO109I - PpuMI (22) Bsu36I (41) I-CeuI (44) BsrBI (53) EagI - NotI (54) BtgI (57) SacII (60) BglI - SfiI (68) EcoRV (76) ApoI - EcoRI (80) AscI - BssHII (87) SalI (94) AccI (95) HincII (96) AflIII - PciI (156) NspI (160) DrdI (264) BciVI (359) HaeII (404) BseYI (460) PspFI (464) AlwNI (572) BanI (835) pYX-Asc 1691 bp
SspI  (0)
1 site
A A T A T T T T A T A A
EarI  (1682)
1 site
C T C T T C N G A G A A G N N N N

Efficient cleavage requires at least two copies of the EarI
recognition sequence.
Sticky ends from different EarI sites may not be compatible.
XmnI  (1486)
1 site
G A A N N N N T T C C T T N N N N A A G
DraI  (1464)
1 site
T T T A A A A A A T T T
BsaHI  (1424)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
ScaI  (1367)
1 site
A G T A C T T C A T G A
TatI  (1365)
1 site
W G T A C W W C A T G W
TsoI  (1286)
1 site
T A R C C A ( N ) 9 N N A T Y G G T ( N ) 9

Sticky ends from different TsoI sites may not be compatible.
After cleavage, TsoI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuI  (1257)
1 site
C G A T C G G C T A G C
FspI  (1109)
1 site
T G C G C A A C G C G T
NmeAIII  (1035)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII
recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BsrFI  (967)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI  (957)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BsaI  (948)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BmrI  (927)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the
absence of magnesium.
AhdI  (887)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
PacI  (6)
1 site
T T A A T T A A A A T T A A T T
EcoO109I  (22)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PpuMI  (22)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
Bsu36I  (41)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
I-CeuI  (44)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
BsrBI  (53)
1 site
C C G C T C G G C G A G

This recognition sequence is asymmetric, so ligating blunt ends
generated by BsrBI will not always regenerate a BsrBI site.
BsrBI is typically used at 37°C, but can be used at temperatures
up to 50°C.
EagI  (54)
1 site
C G G C C G G C C G G C
NotI  (54)
1 site
G C G G C C G C C G C C G G C G
BtgI  (57)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
SacII  (60)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
BglI  (68)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
SfiI  (68)
1 site
G G C C N N N N N G G C C C C G G N N N N N C C G G

Efficient cleavage requires at least two copies of the SfiI
recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
EcoRV  (76)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
ApoI  (80)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
EcoRI  (80)
1 site
G A A T T C C T T A A G
AscI  (87)
1 site
G G C G C G C C C C G C G C G G
BssHII  (87)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
SalI  (94)
1 site
G T C G A C C A G C T G
AccI  (95)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
HincII  (96)
1 site
G T Y R A C C A R Y T G
AflIII  (156)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
PciI  (156)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
NspI  (160)
1 site
R C A T G Y Y G T A C R
DrdI  (264)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
BciVI  (359)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
HaeII  (404)
1 site
R G C G C Y Y C G C G R
BseYI  (460)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (464)
1 site
C C C A G C G G G T C G
AlwNI  (572)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BanI  (835)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
AmpR
814 .. 1674  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   814 .. 1605  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
814 .. 1674  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   1606 .. 1674  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
814 .. 1674  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
ori
217 .. 805  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
217 .. 805  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
MCS
40 .. 99  =  60 bp
multiple cloning site
MCS
40 .. 99  =  60 bp
multiple cloning site
T3 promoter
4 .. 22  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T3 promoter
4 .. 22  =  19 bp
promoter for bacteriophage T3 RNA polymerase
T7 promoter
137 .. 155  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
137 .. 155  =  19 bp
promoter for bacteriophage T7 RNA polymerase
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