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Plasmid Files

BaculoDirect™ C-Term Linear DNA

Gateway® destination vector consisting of a modified baculovirus genome, for expressing C-terminally V5-6xHis-tagged proteins.

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BaculoDirect C-Term Linear DNA Sequence and MapBaculoDirect C-Term Linear DNA.dna
Map and Sequence File   
Sequence Author:  Invitrogen (Life Technologies)
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 polyhedrin promoter attR1 HSV TK poly(A) signal IE0 promoter SbfI (139,013) SrfI (139,018) p10 promoter < Bsu36I > (139,370) < Bsu36I > (0) MauBI (1274) V5 tag 6xHis AvrII (10,132) AbsI (24,309) FseI (32,539) BaculoDirect™ C-Term Linear DNA 139,370 bp
SbfI  (139,013)
1 site
C C T G C A G G G G A C G T C C
SrfI  (139,018)
1 site
G C C C G G G C C G G G C C C G
End  (139,370)
0 sites
Start  (0)
0 sites
MauBI  (1274)
1 site
C G C G C G C G G C G C G C G C
AvrII  (10,132)
1 site
C C T A G G G G A T C C
AbsI  (24,309)
1 site
C C T C G A G G G G A G C T C C
FseI  (32,539)
1 site
G G C C G G C C C C G G C C G G

FseI gradually loses activity when stored at -20°C.
lacZ
1 .. 2836  =  2836 bp
944 amino acids  =  107.2 kDa
Product: β-galactosidase
lacZ
1 .. 2836  =  2836 bp
944 amino acids  =  107.2 kDa
Product: β-galactosidase
HSV TK
137,265 .. 138,395  =  1131 bp
376 amino acids  =  40.9 kDa
Product: herpes simplex virus thymidine kinase
HSV TK
137,265 .. 138,395  =  1131 bp
376 amino acids  =  40.9 kDa
Product: herpes simplex virus thymidine kinase
IE0 promoter
138,400 .. 138,754  =  355 bp
promoter of the ie0 gene from the baculovirus
Autographa californica
IE0 promoter
138,400 .. 138,754  =  355 bp
promoter of the ie0 gene from the baculovirus
Autographa californica
lacZ
139,132 .. 139,373  =  242 bp
80 amino acids  =  9.3 kDa
Product: β-galactosidase
lacZ
139,132 .. 139,373  =  242 bp
80 amino acids  =  9.3 kDa
Product: β-galactosidase
attR2
2852 .. 2976  =  125 bp
recombination site for the Gateway® LR reaction
attR2
2852 .. 2976  =  125 bp
recombination site for the Gateway® LR reaction
attR1
136,854 .. 136,978  =  125 bp
recombination site for the Gateway® LR reaction
attR1
136,854 .. 136,978  =  125 bp
recombination site for the Gateway® LR reaction
p10 promoter
139,023 .. 139,121  =  99 bp
baculovirus promoter for expression in insect cells
p10 promoter
139,023 .. 139,121  =  99 bp
baculovirus promoter for expression in insect cells
polyhedrin promoter
136,705 .. 136,796  =  92 bp
promoter for the baculovirus polyhedrin gene
polyhedrin promoter
136,705 .. 136,796  =  92 bp
promoter for the baculovirus polyhedrin gene
HSV TK poly(A) signal
137,175 .. 137,223  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
HSV TK poly(A) signal
137,175 .. 137,223  =  49 bp
herpesvirus thymidine kinase polyadenylation signal
V5 tag
2996 .. 3037  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
V5 tag
2996 .. 3037  =  42 bp
14 amino acids  =  1.4 kDa
Product: epitope tag from simian virus 5
6xHis
3047 .. 3064  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
3047 .. 3064  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
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