Resources
Plasmid Files

pAB-bee™-FH

Baculovirus transfer plasmid for high-level expression of secreted proteins with a C-terminal FLAG®-8xHis cassette.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pAB-bee-FH.dna
Map and Sequence File:    Download    Open   
Sequence Author:  AB Vector
Download Free Trial Get SnapGene Viewer


NdeI (9569) ScaI (8878) BpmI (8468) AlwNI (7921) PspFI (7813) BseYI (7809) BspQI - SapI (7389) FspAI (6472) SgrDI (6319) SgrAI (6050) SnaBI (4962) KpnI (4782) PasI (486) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) PaeR7I - PspXI - XhoI (1901) SphI (2135) BclI * (2232) BbvCI - Bpu10I (3405) NgoMIV (3770) NaeI (3772) EcoRV (4000) BglII (4134) BstZ17I (4184) EagI - NotI (4204) EcoRI (4215) TspMI - XmaI (4220) SmaI (4222) BamHI (4224) XbaI (4232) AvrII (4306) KflI - PpuMI (4331) Acc65I (4778) pAB-bee™-FH 9783 bp
NdeI  (9569)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
ScaI  (8878)
1 site
A G T A C T T C A T G A
BpmI  (8468)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
AlwNI  (7921)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7813)
1 site
C C C A G C G G G T C G
BseYI  (7809)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (7389)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7389)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
FspAI  (6472)
1 site
R T G C G C A Y Y A C G C