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Plasmid Files

pAB-bee™

Baculovirus transfer plasmid for high-level expression of secreted proteins with a C-terminal 8xHis tag.

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pAB-bee.dna
Map and Sequence File:    Download    Open   
Sequence Author:  AB Vector
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NdeI (9718) ScaI (9027) AlwNI (8070) PspFI (7962) BseYI (7958) BspQI - SapI (7538) AanI (6910) FspAI (6621) SgrDI (6468) AanI (6279) SgrAI (6199) AanI (5136) SnaBI (5111) AanI (121) PacI (583) XcmI (746) BtgI (868) SacII (871) BstEII (923) PspOMI (1395) ApaI - BanII (1399) PaeR7I - PspXI - XhoI (1901) SphI (2135) BclI * (2232) AanI (3309) AanI (3398) BbvCI - Bpu10I (3405) NgoMIV (3770) NaeI (3772) EcoRV (4000) BglII (4134) BstZ17I (4184) EagI - NotI (4204) EcoRI (4215) XbaI (4219) TspMI - XmaI (4230) SmaI (4232) BamHI (4234) EcoRI (4406) AvrII (4455) PpuMI - SanDI (4480) pAB-bee™ 9932 bp
NdeI  (9718)
1 site
C A T A T G G T A T A C

Prolonged incubation with NdeI may lead to removal of additional nucleotides.
ScaI  (9027)
1 site
A G T A C T T C A T G A
AlwNI  (8070)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (7962)
1 site
C C C A G C G G G T C G
BseYI  (7958)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BspQI  (7538)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (7538)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
AanI  (6910)
6 sites
T T A T A A A A T A T T
FspAI  (6621)
1 site
R T G C G C A Y Y A C G C G T R
SgrDI  (6468)
1 site
C G T C G A C G G C A G C T G C
AanI  (6279)
6 sites
T T A T A A A A T A T T
SgrAI  (6199)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
AanI  (5136)
6 sites
T T A T A A A A T A T T
SnaBI  (5111)
1 site
T A C G T A A T G C A T
AanI  (121)
6 sites
T T A T A A A A T A T T
PacI  (583)
1 site
T T A A T T A A A A T T A A T T
XcmI  (746)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
BtgI  (868)
1 site
C C R Y G G G G Y R C C

Sticky ends from different BtgI sites may not be compatible.
SacII  (871)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BstEII  (923)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
PspOMI  (1395)
1 site
G G G C C C C C C G G G
ApaI  (1399)
1 site
G G G C C C C C C G G G

ApaI can be used between 25°C and 37°C.
BanII  (1399)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PaeR7I  (1901)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1901)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1901)
1 site
C T C G A G G A G C T C
SphI  (2135)
1 site
G C A T G C C G T A C G
BclI  (2232)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
AanI  (3309)
6 sites
T T A T A A A A T A T T
AanI  (3398)
6 sites
T T A T A A A A T A T T
BbvCI  (3405)
1 site
C C T C A G C G G A G T C G
Bpu10I  (3405)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
NgoMIV  (3770)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV recognition sequence.
NaeI  (3772)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI recognition sequence.
EcoRV  (4000)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BglII  (4134)
1 site
A G A T C T T C T A G A
BstZ17I  (4184)
1 site
G T A T A C C A T A T G
EagI  (4204)
1 site
C G G C C G G C C G G C
NotI  (4204)
1 site
G C G G C C G C C G C C G G C G
EcoRI  (4215)
2 sites
G A A T T C C T T A A G
XbaI  (4219)
1 site
T C T A G A A G A T C T
TspMI  (4230)
1 site
C C C G G G G G G C C C
XmaI  (4230)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (4232)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
BamHI  (4234)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (4406)
2 sites
G A A T T C C T T A A G
AvrII  (4455)
1 site
C C T A G G G G A T C C
PpuMI  (4480)
1 site
R G G W C C Y Y C C W G G R

Sticky ends from different PpuMI sites may not be compatible.
SanDI  (4480)
1 site
G G G W C C C C C C W G G G

Sticky ends from different SanDI sites may not be compatible.
AmpR
8474 .. 9334  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   8474 .. 9265  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8474 .. 9334  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   9266 .. 9334  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
AmpR
8474 .. 9334  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics
baculovirus recombination region (lef2/ORF603)
3170 .. 3997  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
3170 .. 3997  =  828 bp
contains ORF603 and part of lef2