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pACEBac2

Baculovirus transfer vector with a p10 promoter and a gentamycin resistance marker. Serves as an acceptor vector in the MultiBac™ system.

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pACEBac2 Sequence and MappACEBac2.dna
Map and Sequence File   
Sequence Author:  Geneva Biotech
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 BstXI (2591) AseI - PacI (2496) BbsI - BclI * (2437) SmaI (2433) TspMI - XmaI (2431) PaeR7I - XhoI (2423) NcoI (2417) BmtI (2414) NheI (2410) PvuII (2407) NsiI (2403) SphI (2396) KpnI (2390) Acc65I - BanI (2386) BsaI (2224) BanII (2146) PmeI (2090) I-CeuI (2078) Bsu36I (2075) loxP DraIII (1984) BsrGI - TatI (1811) PflFI - Tth111I (1629) BsmBI (1583) BtgZI (1519) BsaBI * (1417) BsrDI (1376) AgeI - BsrFI (1) BspHI (114) AlwNI (425) ApaLI (520) BaeGI - Bme1580I (524) BseYI (530) PspFI (534) BciVI (636) BssS α I (661) BspQI - SapI (881) FspI (890) MscI (1111) EagI - SacII (1169) EcoRV (1226) BsaAI (1307) BseRI (1362) pACEBac2 2761 bp
BstXI  (2591)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
AseI  (2496)
1 site
A T T A A T T A A T T A
PacI  (2496)
1 site
T T A A T T A A A A T T A A T T
BbsI  (2437)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
BclI  (2437)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
SmaI  (2433)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
TspMI  (2431)
1 site
C C C G G G G G G C C C
XmaI  (2431)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
PaeR7I  (2423)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2423)
1 site
C T C G A G G A G C T C
NcoI  (2417)
1 site
C C A T G G G G T A C C
BmtI  (2414)
1 site
G C T A G C C G A T C G
NheI  (2410)
1 site
G C T A G C C G A T C G
PvuII  (2407)
1 site
C A G C T G G T C G A C
NsiI  (2403)
1 site
A T G C A T T A C G T A
SphI  (2396)
1 site
G C A T G C C G T A C G
KpnI  (2390)
1 site
G G T A C C C C A T G G
Acc65I  (2386)
1 site
G G T A C C C C A T G G
BanI  (2386)
1 site
G G Y R C C C C R Y G G

Sticky ends from different BanI sites may not be compatible.
BsaI  (2224)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BanII  (2146)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PmeI  (2090)
1 site
G T T T A A A C C A A A T T T G
I-CeuI  (2078)
1 site
T A A C T A T A A C G G T C C T A A G G T A G C G A A T T G A T A T T G C C A G G A T T C C A T C G C T

I-CeuI is a homing endonuclease that can recognize a variety of
similar recognition sequences.
Bsu36I  (2075)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
DraIII  (1984)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsrGI  (1811)
1 site
T G T A C A A C A T G T

BsrGI is typically used at 37°C, but is even more active at 60°C.
TatI  (1811)
1 site
W G T A C W W C A T G W
PflFI  (1629)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (1629)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BsmBI  (1583)
1 site
C G T C T C N G C A G A G N ( N ) 4

Sticky ends from different BsmBI sites may not be compatible.
BtgZI  (1519)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
BsaBI  (1417)
1 site
G A T N N N N A T C C T A N N N N T A G
* Blocked by Dam methylation.
BsrDI  (1376)
1 site
G C A A T G N N C G T T A C

Sticky ends from different BsrDI sites may not be compatible.
AgeI  (1)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BsrFI  (1)
1 site
R C C G G Y Y G G C C R

Efficient cleavage requires at least two copies of the BsrFI
recognition sequence.
After cleavage, BsrFI can remain bound to DNA and alter its
electrophoretic mobility.
BspHI  (114)
1 site
T C A T G A A G T A C T
AlwNI  (425)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
ApaLI  (520)
1 site
G T G C A C C A C G T G
BaeGI  (524)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (524)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BseYI  (530)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (534)
1 site
C C C A G C G G G T C G
BciVI  (636)
1 site
G T A T C C ( N ) 5 N C A T A G G ( N ) 5

The 1-base overhangs produced by BciVI may be hard to ligate.
Sticky ends from different BciVI sites may not be compatible.
BssSαI  (661)
1 site
C A C G A G G T G C T C
BspQI  (881)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (881)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be
mixed before removing an aliquot.
FspI  (890)
1 site
T G C G C A A C G C G T
MscI  (1111)
1 site
T G G C C A A C C G G T
EagI  (1169)
1 site
C G G C C G G C C G G C
SacII  (1169)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII
recognition sequence.
EcoRV  (1226)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
BsaAI  (1307)
1 site
Y A C G T R R T G C A Y
BseRI  (1362)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
ori
190 .. 778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
190 .. 778  =  589 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
GmR
1203 .. 1736  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
GmR
1203 .. 1736  =  534 bp
177 amino acids  =  19.4 kDa
Product: gentamycin acetyltransferase
confers resistance to gentamycin
Tn7R
913 .. 1136  =  224 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7R
913 .. 1136  =  224 bp
mini-Tn7 element (right end of the Tn7 transposon)
Tn7L
2596 .. 2761  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
Tn7L
2596 .. 2761  =  166 bp
mini-Tn7 element (left end of the Tn7 transposon)
p10 promoter
2457 .. 2566  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
2457 .. 2566  =  110 bp
baculovirus promoter for expression in insect cells
MCS
2386 .. 2449  =  64 bp
multiple cloning site
MCS
2386 .. 2449  =  64 bp
multiple cloning site
HSV TK poly(A) signal
2273 .. 2321  =  49 bp
herpes simplex virus thymidine kinase
polyadenylation signal
HSV TK poly(A) signal
2273 .. 2321  =  49 bp
herpes simplex virus thymidine kinase
polyadenylation signal
loxP
2024 .. 2057  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
2024 .. 2057  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
Pc promoter
1925 .. 1953  =  29 bp
   Segment 3:  -10  
   1925 .. 1930  =  6 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
   Segment 2:  
   1931 .. 1947  =  17 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
   Segment 1:  -35  
   1948 .. 1953  =  6 bp
class 1 integron promoter
Pc promoter
1925 .. 1953  =  29 bp
3 segments
class 1 integron promoter
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