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pBAC™-1

Baculovirus transfer plasmid with the polyhedrin promoter, encoding an optional C-terminal 6xHis tag.

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pBAC-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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BsmI (85) DraIII (4967) ScaI (4295) NmeAIII (3963) BpmI (3885) BmrI (3855) AlwNI (3338) PspFI (3230) BseYI (3226) PciI (2922) PmeI (2802) SgrAI (2514) BstXI (315) NsiI (332) MluI (547) BbvCI - Bpu10I (564) BglII (1161) BsaBI (1192) BamHI (1263) StuI * (1271) EcoRI (1275) Eco53kI (1283) SacI (1285) HindIII (1288) EagI - NotI (1295) AvaI - BsoBI - PaeR7I - PspXI - XhoI (1303) BmeT110I (1304) AvrII - StyI (1330) BlpI (1339) SphI (1349) SnaBI (1426) SwaI (1516) ZraI (1705) AatII (1707) BmgBI (1930) AgeI (2274) AleI (2505) pBAC™-1 5259 bp
BsmI  (85)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
DraIII  (4967)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (4295)
1 site
A G T A C T T C A T G A
NmeAIII  (3963)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
BpmI  (3885)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BmrI  (3855)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
AlwNI  (3338)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
PspFI  (3230)
1 site
C C C A G C G G G T C G
BseYI  (3226)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
PciI  (2922)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PmeI  (2802)
1 site
G T T T A A A C C A A A T T T G
SgrAI  (2514)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BstXI  (315)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NsiI  (332)
1 site
A T G C A T T A C G T A
MluI  (547)
1 site
A C G C G T T G C G C A
BbvCI  (564)
1 site
C C T C A G C G G A G T C G
Bpu10I  (564)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BglII  (1161)
1 site
A G A T C T T C T A G A
BsaBI  (1192)
1 site
G A T N N N N A T C C T A N N N N T A G
BamHI  (1263)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
StuI  (1271)
1 site
A G G C C T T C C G G A
* Blocked by Dcm methylation.
EcoRI  (1275)
1 site
G A A T T C C T T A A G
Eco53kI  (1283)
1 site
G A G C T C C T C G A G
SacI  (1285)
1 site
G A G C T C C T C G A G
HindIII  (1288)
1 site
A A G C T T T T C G A A
EagI  (1295)
1 site
C G G C C G G C C G G C
NotI  (1295)
1 site
G C G G C C G C C G C C G G C G
AvaI  (1303)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (1303)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (1303)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1303)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1303)
1 site
C T C G A G G A G C T C
BmeT110I  (1304)
1 site
C Y C G R G G R G C Y C
AvrII  (1330)
1 site
C C T A G G G G A T C C
StyI  (1330)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
BlpI  (1339)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
SphI  (1349)
1 site
G C A T G C C G T A C G
SnaBI  (1426)
1 site
T A C G T A A T G C A T
SwaI  (1516)
1 site
A T T<