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Plasmid Files

pBACgus-1

Baculovirus transfer plasmid with the polyhedrin promoter, encoding β-glucuronidase plus an optional C-terminal 6xHis tag.

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pBACgus-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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DraIII (7116) ScaI (6444) NmeAIII (6112) BpmI (6034) PspFI (5379) BseYI (5375) PmeI (4951) SgrAI (4663) AgeI (4423) BmgBI (4079) AatII (3856) ZraI (3854) BstXI (315) NsiI (332) BbvCI - Bpu10I (564) BstBI (1882) MscI (2356) PshAI (2404) BtgI (2411) BglII (3310) BamHI (3412) StuI * (3420) EcoRI (3424) Eco53kI (3432) SacI (3434) HindIII (3437) EagI - NotI (3444) AvaI - BsoBI - PaeR7I - PspXI - XhoI (3452) BmeT110I (3453) AvrII - StyI (3479) BlpI (3488) SphI (3498) SwaI (3665) pBACgus-1 7408 bp
DraIII  (7116)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
ScaI  (6444)
1 site
A G T A C T T C A T G A