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Plasmid Files

pBACsurf-1

Baculovirus transfer plasmid with the polyhedrin promoter, for expressing GP64 fusion proteins anchored in the membrane of the baculovirus nucleocapsid.

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pBACsurf-1.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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PspFI (8770) BseYI (8766) BpmI (8113) NmeAIII (8035) ScaI (7701) EcoO109I (7202) PfoI (7143) DraIII (6786) BanII (6712) SgrDI (5249) SgrAI (4970) BclI * (331) BstXI (1255) EcoRV (2099) SpeI (2205) BfuAI - BspMI (2310) PstI (2321) Acc65I (2322) KpnI (2326) TspMI - XmaI (2327) SmaI (2329) NruI (2660) SacII (2676) PaeR7I - PspXI - XhoI (2794) BstZ17I (2829) NotI (3106) PflFI - Tth111I (3517) PflMI (3584) SnaBI (3882) SwaI (3972) AgeI (4730) pBACsurf-1 9429 bp
PspFI  (8770)
1 site
C C C A G C G G G T C G
BseYI  (8766)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
BpmI  (8113)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
NmeAIII  (8035)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
ScaI  (7701)
1 site
A G T A C T T C A T G A
EcoO109I  (7202)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (7143)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
DraIII  (6786)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BanII  (6712)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
SgrDI  (5249)
1 site
C G T C G A C G G C A G C T G C
SgrAI  (4970)
1 site
C R C C G G Y G G Y G G C C R C

Efficient cleavage requires at least two copies of the SgrAI recognition sequence.
BclI  (331)
1 site
T G A T C A A C T A G T
* Blocked by Dam methylation.
BclI is typically used at 50-55°C, but is 50% active at 37°C.
BstXI  (1255)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
EcoRV  (2099)
1 site
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