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pBacPAK8

Baculovirus vector for strong constitutive expression of proteins in insect cells. For an alternative MCS, use pBacPAK9.

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pBacPAK8.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Clontech (TaKaRa)
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BspQI - SapI (5475) PciI (5358) PspFI (5058) BseYI (5054) AlwNI (4949) BmrI (4430) BpmI (4401) BglI (4352) NmeAIII (4323) PvuI (4101) ScaI (3989) EcoO109I (3490) PfoI (3431) DraIII (3074) BsmI (83) BstXI (313) NsiI (330) MluI (545) BbvCI - Bpu10I (562) BseRI (678) EcoRV (1157) Bac1 Primer (1181 .. 1200) BsaBI (1182) BamHI (1252) PstI - SbfI (1262) StuI (1264) PaeR7I - PspXI - XhoI (1266) BstBI (1273) XbaI (1278) BglII (1284) Acc65I (1290) KpnI (1294) Eco53kI (1298) SacI (1300) EcoRI (1302) TspMI - XmaI (1307) SmaI (1309) EagI - NotI (1313) PacI (1324) Bac2 Primer (1355 .. 1374) SnaBI (1400) SwaI (1490) HindIII (1763) BmgBI (1904) AgeI (2248) AleI (2479) SgrAI (2488) pBacPAK8 5538 bp
BspQI  (5475)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5475)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (5358)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
PspFI  (5058)
1 site
C C C A G C G G G T C G
BseYI  (5054)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
AlwNI  (4949)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
BmrI  (4430)
1 site
A C T G G G ( N ) 4 N T G A C C C ( N ) 4

The 1-base overhangs produced by BmrI may be hard to ligate.
Sticky ends from different BmrI sites may not be compatible.
Unlike most restriction enzymes, BmrI can cleave DNA in the absence of magnesium.
BpmI  (4401)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BglI  (4352)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
NmeAIII  (4323)
1 site
G C C G A G ( N ) 18-19 N N C G G C T C ( N ) 18-19

Efficient cleavage requires at least two copies of the NmeAIII recognition sequence.
Sticky ends from different NmeAIII sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
PvuI  (4101)
1 site
C G A T C G G C T A G C
ScaI  (3989)
1 site
A G T A C T T C A T G A
EcoO109I  (3490)
1 site
R G G N C C Y Y C C N G G R

Sticky ends from different EcoO109I sites may not be compatible.
PfoI  (3431)
1 site
T C C N G G A A G G N C C T

Sticky ends from different PfoI sites may not be compatible.
DraIII  (3074)
1 site
C A C N N N G T G G T G N N N C A C

Sticky ends from different DraIII sites may not be compatible.
BsmI  (83)
1 site
G A A T G C N C T T A C G N

Sticky ends from different BsmI sites may not be compatible.
BstXI  (313)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
NsiI  (330)
1 site
A T G C A T T A C G T A
MluI  (545)
1 site
A C G C G T T G C G C A
BbvCI  (562)
1 site
C C T C A G C G G A G T C G
Bpu10I  (562)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BseRI  (678)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
EcoRV  (1157)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BsaBI  (1182)
1 site
G A T N N N N A T C C T A N N N N T A G
BamHI  (1252)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
PstI  (1262)
1 site
C T G C A G G A C G T C
SbfI  (1262)
1 site
C C T G C A G G G G A C G T C C
StuI  (1264)
1 site
A G G C C T T C C G G A
PaeR7I  (1266)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
PspXI  (1266)
1 site
V C T C G A G B B G A G C T C V
XhoI  (1266)
1 site
C T C G A G G A G C T C
BstBI  (1273)
1 site
T T C G A A A A G C T T
XbaI  (1278)
1 site
T C T A G A A G A T C T
BglII  (1284)
1 site
A G A T C T T C T A G A
Acc65I  (1290)
1 site
G G T A C C C C A T G G
KpnI  (1294)
1 site
G G T A C C C C A T G G
Eco53kI  (1298)
1 site
G A G C T C C T C G A G
SacI  (1300)
1 site
G A G C T C C T C G A G
EcoRI  (1302)
1 site
G A A T T C C T T A A G
TspMI  (1307)
1 site
C C C G G G G G G C C C
XmaI  (1307)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1309)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
EagI  (1313)
1 site
C G G C C G G C C G G C
NotI  (1313)
1 site
G C G G C C G C C G C C G G C G
PacI  (1324)
1 site
T T A A T T A A A A T T A A T T
SnaBI  (1400)
1 site
T A C G T A A T G C A T
SwaI  (1490)
1 site
A T