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Plasmid Files

pBiEx™-3

Vector for high-level expression in both bacterial and insect cells of proteins with a C-terminal S-Tag-8xHis cassette.

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pBiEx-3.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Novagen (EMD Millipore)
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TspMI - XmaI (5368) BspQI - SapI (5124) PciI (5007) DrdI (4905) AlwNI (4598) AhdI (4119) BsaI (4053) ScaI (3638) XmnI (3519) EcoNI (3130) PfoI (3094) BstAPI (2986) BstEII (2480) SmaI (5370) BspDI * - ClaI * (207) PsiI (409) NheI (606) BmtI (610) SphI (614) BsmI (775) BseRI (781) BsiWI (784) SacII (1085) NcoI (1177) BmgBI (1194) Eco53kI (1202) SacI (1204) BamHI (1208) MfeI (1214) BglII (1222) BfuAI - BspMI (1229) AscI (1230) PstI - SbfI (1240) SalI (1242) AccI (1243) Acc65I (1248) AgeI (1251) KpnI (1252) BstBI (1257) HindIII (1260) NotI (1267) PaeR7I - PspXI - XhoI (1344) DraIII (1376) BbvCI - Bpu10I (1386) BlpI (1423) EcoO109I (1450) Bsu36I (1500) PacI (1529) PflFI - Tth111I (1753) KasI (2023) NarI * (2024) SfoI (2025) PluTI (2027) BsmBI (2047) PspOMI (2455) ApaI (2459) pBiEx™-3 5373 bp
TspMI  (5368)
1 site
C C C G G G G G G C C C
XmaI  (5368)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
BspQI  (5124)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5124)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PciI  (5007)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
DrdI  (4905)
1 site
G A C N N N N N N G T C C T G N N N N N N C A G

Sticky ends from different DrdI sites may not be compatible.
AlwNI  (4598)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (4119)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaI  (4053)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
ScaI  (3638)
1 site
A G T A C T