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pDEST™10

Gateway® destination vector for expressing N-terminally 6xHis-tagged proteins in insect cells using the Bac-to-Bac® baculovirus system.

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pDEST10.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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BbsI (6677) PflFI - Tth111I (6148) BseRI (5881) SacII (5688) BspQI - SapI (5301) PspFI (4884) BseYI (4880) FspI (4073) PvuI (3927) BsaHI (3756) SnaBI (93) RsrII (251) PvuII (824) MluI (1371) BssHII (1418) BbvCI (1649) TspMI - XmaI (1793) SmaI - SrfI (1795) BmgBI (1829) BfuAI - BspMI (2039) StuI (2208) SgrDI (2214) Eco53kI (2222) SacI (2224) SpeI (2227) BstBI (2243) XbaI (2248) PaeR7I - XhoI (2263) SphI (2273) Acc65I (2275) KpnI (2279) HindIII (2281) MfeI (2396) HpaI (2409) BclI * (2545) AvrII (2560) NgoMIV (3047) NaeI (3049) pDEST™10 6708 bp
BbsI  (6677)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
PflFI  (6148)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by PflFI may be hard to ligate.
Sticky ends from different PflFI sites may not be compatible.
Tth111I  (6148)
1 site
G A C N N N G T C C T G N N N C A G

The 1-base overhangs produced by Tth111I may be hard to ligate.
Sticky ends from different Tth111I sites may not be compatible.
BseRI  (5881)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the DNA.
SacII  (5688)
1 site
C C G C G G G G C G C C

Efficient cleavage requires at least two copies of the SacII recognition sequence.
BspQI  (5301)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different BspQI sites may not be compatible.
SapI  (5301)
1 site
G C T C T T C N C G A G A A G N N N N

Sticky ends from different SapI sites may not be compatible.
SapI gradually settles in solution, so a tube of SapI should be mixed before removing an aliquot.
PspFI  (4884)
1 site
C C C A G C G G G T C G
BseYI  (4880)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its electrophoretic mobility.
FspI  (4073)
1 site
T G C G C A A C G C G T
PvuI  (3927)
1 site
C G A T C G G C T A G C
BsaHI  (3756)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
SnaBI  (93)
1 site
T A C G T A A T G C A T
RsrII  (251)
1 site
C G G W C C G G C C W G G C

Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.
For full activity, add fresh DTT.
PvuII  (824)
1 site
C A G C T G G T C G A C
MluI  (1371)
1 site
A C G C G T T G C G C A
BssHII  (1418)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
BbvCI  (1649)
1 site
C C T C A G C G G A G T C G
TspMI  (1793)
1 site
C C C G G G G G G C C C
XmaI  (1793)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1795)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief in