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pIDS

Baculovirus transfer vector with a p10 promoter and a spectinomycin resistance marker. Serves as a donor vector in the MultiBac™ system.

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pIDS Sequence and MappIDS.dna
Map and Sequence File   
Sequence Author:  Geneva Biotech
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 AvrII (2223) PI-SceI (2198) ApoI (2106) BssHII (2087) MmeI (2080) NaeI (2014) NgoMIV (2012) BglI (1995) BtgZI (1921) EciI (1827) BsiEI - PvuI (1744) BfuAI - BspMI (1710) BlpI (1672) BaeGI - Bme1580I - BsiHKAI (1642) ApaLI (1638) MslI (1635) SfcI (1618) BpmI (1615) BstEII (1523) AcuI (1509) EaeI (1450) BsaHI (1417) BspHI (1363) EcoP15I (1359) AgeI (1131) BspDI - ClaI (0) SpeI (5) AccI (10) BstZ17I (11) PacI (87) BbsI (140) TspMI - XmaI (146) SmaI (148) PaeR7I - SmlI - XhoI (154) NcoI (160) NheI (167) BmtI (171) NsiI (182) NspI - SphI (189) Acc65I (191) KpnI (195) BsaI (353) BanII (439) PmeI (491) BstXI (503) loxP SnaBI (549) PsiI (758) HindIII (789) AflIII (962) BseYI (1013) PspFI (1017) Bpu10I (1068) Bsu36I (1082) pIDS 2231 bp
AvrII  (2223)
1 site
C C T A G G G G A T C C
PI-SceI  (2198)
1 site
A T C T A T G T C G G G T G C G G A G A A A G A G G T A A T G A A A T G G T A G A T A C A G C C C A C G C C T C T T T C T C C A T T A C T T T A C C

PI-SceI  is a homing endonuclease that can recognize a variety of
similar recognition sequences.
ApoI  (2106)
1 site
R A A T T Y Y T T A A R

ApoI is typically used at 50°C, but is 50% active at 37°C.
BssHII  (2087)
1 site
G C G C G C C G C G C G

BssHII is typically used at 50°C, but is 75% active at 37°C.
MmeI  (2080)
1 site
T C C R A C ( N ) 18 N N A G G Y T G ( N ) 18

Efficient cleavage requires at least two copies of the MmeI
recognition sequence.
Sticky ends from different MmeI sites may not be compatible.
For full activity, add fresh S-adenosylmethionine (SAM).
NaeI  (2014)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NaeI
recognition sequence.
NgoMIV  (2012)
1 site
G C C G G C C G G C C G

Efficient cleavage requires at least two copies of the NgoMIV
recognition sequence.
BglI  (1995)
1 site
G C C N N N N N G G C C G G N N N N N C C G

Sticky ends from different BglI sites may not be compatible.
BtgZI  (1921)
1 site
G C G A T G ( N ) 10 C G C T A C ( N ) 10 ( N ) 4

Sticky ends from different BtgZI sites may not be compatible.
After cleavage, BtgZI can remain bound to DNA and alter its
electrophoretic mobility.
BtgZI is typically used at 60°C, but is 75% active at 37°C.
EciI  (1827)
1 site
G G C G G A ( N ) 9 N N C C G C C T ( N ) 9

Sticky ends from different EciI sites may not be compatible.
BsiEI  (1744)
1 site
C G R Y C G G C Y R G C

Sticky ends from different BsiEI sites may not be compatible.
PvuI  (1744)
1 site
C G A T C G G C T A G C
BfuAI  (1710)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1710)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BlpI  (1672)
1 site
G C T N A G C C G A N T C G

Sticky ends from different BlpI sites may not be compatible.
BaeGI  (1642)
1 site
G K G C M C C M C G K G

Sticky ends from different BaeGI sites may not be compatible.
Bme1580I  (1642)
1 site
G K G C M C C M C G K G

Sticky ends from different Bme1580I sites may not be compatible.
BsiHKAI  (1642)
1 site
G W G C W C C W C G W G

Sticky ends from different BsiHKAI sites may not be compatible.
ApaLI  (1638)
1 site
G T G C A C C A C G T G
MslI  (1635)
1 site
C A Y N N N N R T G G T R N N N N Y A C
SfcI  (1618)
1 site
C T R Y A G G A Y R T C

Sticky ends from different SfcI sites may not be compatible.
SfcI quickly loses activity at 37°C, but can be used at 25°C for long
incubations.
BpmI  (1615)
1 site
C T G G A G ( N ) 14 N N G A C C T C ( N ) 14

Efficient cleavage requires at least two copies of the BpmI
recognition sequence.
Sticky ends from different BpmI sites may not be compatible.
After cleavage, BpmI can remain bound to DNA and alter its
electrophoretic mobility.
BpmI quickly loses activity at 37°C.
BstEII  (1523)
1 site
G G T N A C C C C A N T G G

Sticky ends from different BstEII sites may not be compatible.
BstEII is typically used at 60°C, but is 50% active at 37°C.
AcuI  (1509)
1 site
C T G A A G ( N ) 14 N N G A C T T C ( N ) 14

Efficient cleavage requires at least two copies of the AcuI
recognition sequence.
Sticky ends from different AcuI sites may not be compatible.
After cleavage, AcuI can remain bound to DNA and alter its
electrophoretic mobility.
For full activity, add fresh S-adenosylmethionine (SAM).
EaeI  (1450)
1 site
Y G G C C R R C C G G Y
BsaHI  (1417)
1 site
G R C G Y C C Y G C R G

BsaHI is typically used at 37°C, but is even more active at 60°C.
BspHI  (1363)
1 site
T C A T G A A G T A C T
EcoP15I  (1359)
1 site
C A G C A G ( N ) 25 G T C G T C ( N ) 25 N N

Efficient cleavage requires two inversely oriented copies of the
EcoP15I recognition sequence.
Sticky ends from different EcoP15I sites may not be compatible.
EcoP15I requires ATP for activity.
AgeI  (1131)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BspDI  (0)
1 site
A T C G A T T A G C T A
ClaI  (0)
1 site
A T C G A T T A G C T A
SpeI  (5)
1 site
A C T A G T T G A T C A
AccI  (10)
1 site
G T M K A C C A K M T G

Efficient cleavage with AccI requires ≥13 bp on each side of the
recognition sequence.
Sticky ends from different AccI sites may not be compatible.
BstZ17I  (11)
1 site
G T A T A C C A T A T G
PacI  (87)
1 site
T T A A T T A A A A T T A A T T
BbsI  (140)
1 site
G A A G A C N N C T T C T G N N ( N ) 4

Sticky ends from different BbsI sites may not be compatible.
BbsI gradually loses activity when stored at -20°C.
TspMI  (146)
1 site
C C C G G G G G G C C C
XmaI  (146)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (148)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
PaeR7I  (154)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
SmlI  (154)
1 site
C T Y R A G G A R Y T C

Efficient cleavage requires at least two copies of the SmlI
recognition sequence.
Sticky ends from different SmlI sites may not be compatible.
XhoI  (154)
1 site
C T C G A G G A G C T C
NcoI  (160)
1 site
C C A T G G G G T A C C
NheI  (167)
1 site
G C T A G C C G A T C G
BmtI  (171)
1 site
G C T A G C C G A T C G
NsiI  (182)
1 site
A T G C A T T A C G T A
NspI  (189)
1 site
R C A T G Y Y G T A C R
SphI  (189)
1 site
G C A T G C C G T A C G
Acc65I  (191)
1 site
G G T A C C C C A T G G
KpnI  (195)
1 site
G G T A C C C C A T G G
BsaI  (353)
1 site
G G T C T C N C C A G A G N ( N ) 4

Sticky ends from different BsaI sites may not be compatible.
BsaI can be used between 37°C and 50°C.
BanII  (439)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
PmeI  (491)
1 site
G T T T A A A C C A A A T T T G
BstXI  (503)
1 site
C C A N N N N N N T G G G G T N N N N N N A C C

Sticky ends from different BstXI sites may not be compatible.
SnaBI  (549)
1 site
T A C G T A A T G C A T
PsiI  (758)
1 site
T T A T A A A A T A T T
HindIII  (789)
1 site
A A G C T T T T C G A A
AflIII  (962)
1 site
A C R Y G T T G Y R C A

Sticky ends from different AflIII sites may not be compatible.
BseYI  (1013)
1 site
C C C A G C G G G T C G

After cleavage, BseYI can remain bound to DNA and alter its
electrophoretic mobility.
PspFI  (1017)
1 site
C C C A G C G G G T C G
Bpu10I  (1068)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I
recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends
generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
Bsu36I  (1082)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
SmR
1365 .. 2156  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase
(Murphy, 1985)
confers resistance to spectinomycin and
streptomycin
SmR
1365 .. 2156  =  792 bp
263 amino acids  =  29.3 kDa
Product: aminoglycoside adenylyltransferase
(Murphy, 1985)
confers resistance to spectinomycin and
streptomycin
R6K γ ori
546 .. 902  =  357 bp
γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication
R6K γ ori
546 .. 902  =  357 bp
γ replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication
p10 promoter
16 .. 125  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
16 .. 125  =  110 bp
baculovirus promoter for expression in insect cells
MCS
133 .. 196  =  64 bp
multiple cloning site
MCS
133 .. 196  =  64 bp
multiple cloning site
HSV TK poly(A) signal
261 .. 309  =  49 bp
herpes simplex virus thymidine kinase
polyadenylation signal
HSV TK poly(A) signal
261 .. 309  =  49 bp
herpes simplex virus thymidine kinase
polyadenylation signal
loxP
511 .. 544  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
loxP
511 .. 544  =  34 bp
Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT).
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