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pMelBac B

Baculovirus transfer vector for expression and secretion of recombinant proteins. For other reading frames, use pMelBac A or pMelBac C.

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pMelBac B.dna
Map and Sequence File:    Download    Open   
Sequence Author:  Thermo Fisher (Invitrogen)
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EcoRV (3) BstAPI (4724) Bsu36I (4292) AleI (4227) Bpu10I (4011) BfuAI - BspMI (3741) BspDI - ClaI (3693) PciI (3286) AlwNI (2877) AhdI (2398) BsaBI (28) BstZ17I (137) BamHI (158) Eco53kI (166) AvaI - BsoBI - PaeR7I - XhoI (167) BanII - BmeT110I - SacI (168) BglII (171) PstI (179) Acc65I (183) KpnI - NcoI - StyI (187) EcoRI (192) BstBI (196) HindIII (199) SalI (208) BseRI (238) AfeI (347) BsaAI - SnaBI (389) PsiI (414) SwaI (479) BsrGI (719) BbsI (848) BmgBI (893) PfoI (1359) EcoO109I (1418) ScaI (1917) BsrFI (2313) BsaI (2332) pMelBac B 4821 bp
EcoRV  (3)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
BstAPI  (4724)
1 site
G C A N N N N N T G C C G T N N N N N A C G

Sticky ends from different BstAPI sites may not be compatible.
Bsu36I  (4292)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
AleI  (4227)
1 site
C A C N N N N G T G G T G N N N N C A C
Bpu10I  (4011)
1 site
C C T N A G C G G A N T C G

Efficient cleavage requires at least two copies of the Bpu10I recognition sequence.
This recognition sequence is asymmetric, so ligating sticky ends generated by Bpu10I will not always regenerate a Bpu10I site.
Sticky ends from different Bpu10I sites may not be compatible.
BfuAI  (3741)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (3741)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
BspDI  (3693)
1 site
A T C G A T T A G C T A
ClaI  (3693)
1 site
A T C G A T T A G C T A
PciI  (3286)
1 site
A C A T G T T G T A C A

PciI is inhibited by nonionic detergents.
AlwNI  (2877)
1 site
C A G N N N C T G G T C N N N G A C

Sticky ends from different AlwNI sites may not be compatible.
AhdI  (2398)
1 site
G A C N N N N N G T C C T G N N N N N C A G

The 1-base overhangs produced by AhdI may be hard to ligate.
Sticky ends from different AhdI sites may not be compatible.
BsaBI  (28)
1 site
G A T N N N N A T C C T A N N N N T A G
BstZ17I  (137)
1 site
G T A T A C C A T A T G
BamHI  (158)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
Eco53kI  (166)
1 site
G A G C T C C T C G A G
AvaI  (167)
1 site
C Y C G R G G R G C Y C

Sticky ends from different AvaI sites may not be compatible.
BsoBI  (167)
1 site
C Y C G R G G R G C Y C

Sticky ends from different BsoBI sites may not be compatible.
BsoBI is typically used at 37°C, but can be used at temperatures up to 65°C.
PaeR7I  (167)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (167)
1 site
C T C G A G G A G C T C
BanII  (168)
1 site
G R G C Y C C Y C G R G

Sticky ends from different BanII sites may not be compatible.
BmeT110I  (168)
1 site
C Y C G R G G R G C Y C
SacI  (168)
1 site
G A G C T C C T C G A G
BglII  (171)
1 site
A G A T C T T C T A G A
PstI  (179)
1 site
C T G C A G G A C G T C
Acc65I  (183)
1 site
G G T A C C C C A T G G
KpnI  (187)
1 site
G G T A C C C C A T G G
NcoI  (187)
1 site
C C A T G G G G T A C C
StyI  (187)
1 site
C C W W G G G G W W C C

Sticky ends from different StyI sites may not be compatible.
EcoRI  (192)
1 site
G A A T T C C T T A A G
BstBI  (196)
1 site
T T C G A A A A G C T T
HindIII  (199)
1 site
A A G C T T T T C