Resources
Plasmid Files

pQE-TriSystem His-Strep2

Vector for expression in bacterial, insect, and vertebrate cells of proteins with an N-terminal Strep-Tag II tag and a C-terminal 8xHis tag.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pQE-TriSystem His-Strep2 Sequence and MappQE-TriSystem His-Strep2.dna
Map and Sequence File   
Sequence Author:  Qiagen
Download Free Trial Get SnapGene Viewer

 AanI (5199) AanI (5110) AanI (3160) AanI (1111) NcoI (1265) TspMI - XmaI (1307) SmaI (1309) Eco53kI (1314) SacI (1316) BamHI (1320) EcoRI (1326) BglII (1334) BfuAI - BspMI (1341) AscI (1342) PstI - SbfI (1352) Acc65I (1360) KpnI (1364) HindIII (1372) EagI - NotI (1379) PvuII (1389) BstZ17I (1394) PmlI (1399) PaeR7I - XhoI (1441) AanI (2017) pQE-TriSystem His-Strep2 5796 bp
AanI  (5199)
5 sites
T T A T A A A A T A T T
AanI  (5110)
5 sites
T T A T A A A A T A T T
AanI  (3160)
5 sites
T T A T A A A A T A T T
AanI  (1111)
5 sites
T T A T A A A A T A T T
NcoI  (1265)
1 site
C C A T G G G G T A C C
TspMI  (1307)
1 site
C C C G G G G G G C C C
XmaI  (1307)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (1309)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
Eco53kI  (1314)
1 site
G A G C T C C T C G A G
SacI  (1316)
1 site
G A G C T C C T C G A G
BamHI  (1320)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (1326)
1 site
G A A T T C C T T A A G
BglII  (1334)
1 site
A G A T C T T C T A G A
BfuAI  (1341)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BfuAI
recognition sequence.
Sticky ends from different BfuAI sites may not be compatible.
BfuAI is typically used at 50°C, but is 50% active at 37°C.
BspMI  (1341)
1 site
A C C T G C ( N ) 4 T G G A C G ( N ) 4 ( N ) 4

Efficient cleavage requires at least two copies of the BspMI
recognition sequence.
Sticky ends from different BspMI sites may not be compatible.
AscI  (1342)
1 site
G G C G C G C C C C G C G C G G
PstI  (1352)
1 site
C T G C A G G A C G T C
SbfI  (1352)
1 site
C C T G C A G G G G A C G T C C
Acc65I  (1360)
1 site
G G T A C C C C A T G G
KpnI  (1364)
1 site
G G T A C C C C A T G G
HindIII  (1372)
1 site
A A G C T T T T C G A A
EagI  (1379)
1 site
C G G C C G G C C G G C
NotI  (1379)
1 site
G C G G C C G C C G C C G G C G
PvuII  (1389)
1 site
C A G C T G G T C G A C
BstZ17I  (1394)
1 site
G T A T A C C A T A T G
PmlI  (1399)
1 site
C A C G T G G T G C A C

PmlI gradually loses activity when stored at -20°C.
PaeR7I  (1441)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (1441)
1 site
C T C G A G G A G C T C
AanI  (2017)
5 sites
T T A T A A A A T A T T
baculovirus recombination region (ORF1629)
1828 .. 3286  =  1459 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
1828 .. 3286  =  1459 bp
contains part of ORF1629
AmpR
4045 .. 4905  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   4045 .. 4836  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4045 .. 4905  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   4837 .. 4905  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
4045 .. 4905  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
baculovirus recombination region (lef2/ORF603)
4971 .. 2  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
4971 .. 2  =  828 bp
contains ORF603 and part of lef2
Exon 2
1005 .. 1649  =  645 bp
Exon 2
1005 .. 1649  =  645 bp
ori
3445 .. 4032  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
3445 .. 4032  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
62 .. 367  =  306 bp
human cytomegalovirus (CMV) immediate early
enhancer
CMV enhancer
62 .. 367  =  306 bp
human cytomegalovirus (CMV) immediate early
enhancer
chicken β-actin promoter
373 .. 650  =  278 bp
chicken β-actin promoter
373 .. 650  =  278 bp
Intron
743 .. 1004  =  262 bp
Intron
743 .. 1004  =  262 bp
Exon 1
651 .. 742  =  92 bp
Exon 1
651 .. 742  =  92 bp
T7 terminator
1768 .. 1815  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
1768 .. 1815  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
ORF1629
1945 .. 3286  =  1342 bp
446 amino acids  =  49.2 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
1945 .. 3286  =  1342 bp
446 amino acids  =  49.2 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF603
5133 .. 5738  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
5133 .. 5738  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
MCS
1307 .. 1446  =  140 bp
multiple cloning site
MCS
1307 .. 1446  =  140 bp
multiple cloning site
lef2
4971 .. 5095  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
4971 .. 5095  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
rabbit globin terminator
1647 .. 1758  =  112 bp
rabbit globin terminator
1647 .. 1758  =  112 bp
p10 promoter
1143 .. 1252  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
1143 .. 1252  =  110 bp
baculovirus promoter for expression in insect cells
T5 promoter
1073 .. 1116  =  44 bp
   Segment 1:  
   1073 .. 1086  =  14 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
   Segment 2:  -35  
   1087 .. 1092  =  6 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
   Segment 3:  
   1093 .. 1109  =  17 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
   Segment 4:  -10  
   1110 .. 1116  =  7 bp
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
T5 promoter
1073 .. 1116  =  44 bp
4 segments
bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator
Strep-Tag II
1279 .. 1302  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin, an
engineered form of streptavidin
Strep-Tag II
1279 .. 1302  =  24 bp
8 amino acids  =  1.1 kDa
Product: peptide that binds Strep-Tactin, an
engineered form of streptavidin
8xHis
1447 .. 1470  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
1447 .. 1470  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
stop codons
1471 .. 1481  =  11 bp
stop codons in all three reading frames
stop codons
1471 .. 1481  =  11 bp
stop codons in all three reading frames
RBS
1255 .. 1260  =  6 bp
ribosome binding site
RBS
1255 .. 1260  =  6 bp
ribosome binding site
poly(A) signal
1625 .. 1630  =  6 bp
poly(A) signal
1625 .. 1630  =  6 bp
ATG
1267 .. 1269  =  3 bp
1 amino acid  =  149.2 Da
start codon
ATG
1267 .. 1269  =  3 bp
1 amino acid  =  149.2 Da
start codon
HSV tag
1408 .. 1440  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
HSV tag
1408 .. 1440  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
lac operator
1092 .. 1108  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
1092 .. 1108  =  17 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter