Resources
Plasmid Files

pTriEx™-2 Neo

Insect, bacterial, and mammalian vector with a neomycin resistance marker, encoding an N-terminal 6xHis-S-Tag cassette and a C-terminal HSV-8xHis cassette.

To see this sequence with restriction sites, features, and translations, please download
 SnapGene or the free  SnapGene Viewer.

pTriEx-2 Neo Sequence and MappTriEx-2 Neo.dna
Map and Sequence File   
Sequence Author:  Novagen (EMD Millipore)
Download Free Trial Get SnapGene Viewer

 AanI (5129) AanI (4572) AanI (320) AanI (409) NcoI (2331) XcmI (2357) TspMI - XmaI (2433) SmaI (2435) PshAI (2475) BseRI (2479) EcoRV (2499) Eco53kI (2506) SacI (2508) BamHI (2512) EcoRI (2518) BglII (2526) AscI (2534) SbfI (2544) AgeI (2555) BstBI (2561) NotI (2571) PvuII (2581) BstZ17I (2586) PaeR7I - XhoI (2633) Bsu36I (2675) pTriEx™-2 Neo 6820 bp
AanI  (5129)
4 sites
T T A T A A A A T A T T
AanI  (4572)
4 sites
T T A T A A A A T A T T
AanI  (320)
4 sites
T T A T A A A A T A T T
AanI  (409)
4 sites
T T A T A A A A T A T T
NcoI  (2331)
1 site
C C A T G G G G T A C C
XcmI  (2357)
1 site
C C A N N N N N N N N N T G G G G T N N N N N N N N N A C C

The 1-base overhangs produced by XcmI may be hard to ligate.
Sticky ends from different XcmI sites may not be compatible.
TspMI  (2433)
1 site
C C C G G G G G G C C C
XmaI  (2433)
1 site
C C C G G G G G G C C C

Efficient cleavage requires at least two copies of the XmaI
recognition sequence.
Full cleavage with XmaI may require a long incubation.
SmaI  (2435)
1 site
C C C G G G G G G C C C

SmaI can be used at 37°C for brief incubations.
PshAI  (2475)
1 site
G A C N N N N G T C C T G N N N N C A G

PshAI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BseRI  (2479)
1 site
G A G G A G ( N ) 8 N N C T C C T C ( N ) 8

Sticky ends from different BseRI sites may not be compatible.
BseRI quickly loses activity at 37°C.
Prolonged incubation with BseRI may lead to degradation of the
DNA.
EcoRV  (2499)
1 site
G A T A T C C T A T A G

EcoRV is reportedly more prone than its isoschizomer Eco32I to
delete a base after cleavage.
Eco53kI  (2506)
1 site
G A G C T C C T C G A G
SacI  (2508)
1 site
G A G C T C C T C G A G
BamHI  (2512)
1 site
G G A T C C C C T A G G

After cleavage, BamHI-HF™ (but not the original BamHI) can
remain bound to DNA and alter its electrophoretic mobility.
EcoRI  (2518)
1 site
G A A T T C C T T A A G
BglII  (2526)
1 site
A G A T C T T C T A G A
AscI  (2534)
1 site
G G C G C G C C C C G C G C G G
SbfI  (2544)
1 site
C C T G C A G G G G A C G T C C
AgeI  (2555)
1 site
A C C G G T T G G C C A

AgeI quickly loses activity at 37°C, but can be used at 25°C for
long incubations.
BstBI  (2561)
1 site
T T C G A A A A G C T T
NotI  (2571)
1 site
G C G G C C G C C G C C G G C G
PvuII  (2581)
1 site
C A G C T G G T C G A C
BstZ17I  (2586)
1 site
G T A T A C C A T A T G
PaeR7I  (2633)
1 site
C T C G A G G A G C T C

PaeR7I does not recognize the sequence CTCTCGAG.
XhoI  (2633)
1 site
C T C G A G G A G C T C
Bsu36I  (2675)
1 site
C C T N A G G G G A N T C C

Sticky ends from different Bsu36I sites may not be compatible.
Exon 2
2082 .. 4204  =  2123 bp
Exon 2
2082 .. 4204  =  2123 bp
AmpR
5898 .. 6758  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 2:  
   5898 .. 6689  =  792 bp
   263 amino acids  =  28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5898 .. 6758  =  861 bp
286 amino acids  =  31.6 kDa
   Segment 1:  signal sequence  
   6690 .. 6758  =  69 bp
   23 amino acids  =  2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
AmpR
5898 .. 6758  =  861 bp
286 amino acids  =  31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and
related antibiotics
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (lef2/ORF603)
181 .. 1008  =  828 bp
contains ORF603 and part of lef2
baculovirus recombination region (ORF1629)
4383 .. 5088  =  706 bp
contains part of ORF1629
baculovirus recombination region (ORF1629)
4383 .. 5088  =  706 bp
contains part of ORF1629
ori
5298 .. 5885  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
ori
5298 .. 5885  =  588 bp
high-copy-number ColE1/pMB1/pBR322/pUC origin
of replication
CMV enhancer
1140 .. 1443  =  304 bp
human cytomegalovirus immediate early enhancer
CMV enhancer
1140 .. 1443  =  304 bp
human cytomegalovirus immediate early enhancer
chicken β-actin promoter
1449 .. 1726  =  278 bp
chicken β-actin promoter
1449 .. 1726  =  278 bp
Intron
1820 .. 2081  =  262 bp
Intron
1820 .. 2081  =  262 bp
Exon 1
1727 .. 1819  =  93 bp
Exon 1
1727 .. 1819  =  93 bp
T7 terminator
4323 .. 4370  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
T7 terminator
4323 .. 4370  =  48 bp
transcription terminator for bacteriophage T7 RNA
polymerase
NeoR/KanR
3259 .. 4062  =  804 bp
267 amino acids  =  29.2 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
NeoR/KanR
3259 .. 4062  =  804 bp
267 amino acids  =  29.2 kDa
Product: aminoglycoside phosphotransferase from
Tn5
confers resistance to neomycin, kanamycin, and
G418 (Geneticin®)
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF603
343 .. 948  =  606 bp
201 amino acids  =  23.6 kDa
Product: baculovirus ORF603 protein
ORF1629
4500 .. 5088  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
ORF1629
4500 .. 5088  =  589 bp
195 amino acids  =  22.3 kDa
Product: baculovirus capsid-associated protein
required for viral replication
IRES
2761 .. 3223  =  463 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
IRES
2761 .. 3223  =  463 bp
internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
lef2
181 .. 305  =  125 bp
40 amino acids  =  4.5 kDa
Product: baculovirus late expression factor 2
MCS
2471 .. 2589  =  119 bp
multiple cloning site
MCS
2471 .. 2589  =  119 bp
multiple cloning site
rabbit globin terminator
4202 .. 4313  =  112 bp
rabbit globin terminator
4202 .. 4313  =  112 bp
p10 promoter
2209 .. 2318  =  110 bp
baculovirus promoter for expression in insect cells
p10 promoter
2209 .. 2318  =  110 bp
baculovirus promoter for expression in insect cells
S-Tag
2366 .. 2410  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from
pancreatic ribonuclease A
S-Tag
2366 .. 2410  =  45 bp
15 amino acids  =  1.7 kDa
Product: affinity and epitope tag derived from
pancreatic ribonuclease A
HSV tag
2600 .. 2632  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
HSV tag
2600 .. 2632  =  33 bp
11 amino acids  =  1.2 kDa
Product: HSV (herpes simplex virus) epitope tag
lac operator
2169 .. 2193  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
lac operator
2169 .. 2193  =  25 bp
The lac repressor binds to the lac operator to inhibit
transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-β-D-thiogalactopyranoside (IPTG).
8xHis
2639 .. 2662  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
8xHis
2639 .. 2662  =  24 bp
8 amino acids  =  1.1 kDa
Product: 8xHis affinity tag
T7 promoter
2150 .. 2168  =  19 bp
promoter for bacteriophage T7 RNA polymerase
T7 promoter
2150 .. 2168  =  19 bp
promoter for bacteriophage T7 RNA polymerase
6xHis
2339 .. 2356  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
6xHis
2339 .. 2356  =  18 bp
6 amino acids  =  840.9 Da
Product: 6xHis affinity tag
thrombin site
2426 .. 2443  =  18 bp
6 amino acids  =  627.8 Da
Product: thrombin recognition and cleavage site
thrombin site
2426 .. 2443  =  18 bp
6 amino acids  =  627.8 Da
Product: thrombin recognition and cleavage site
RBS
2320 .. 2325  =  6 bp
ribosome binding site
RBS
2320 .. 2325  =  6 bp
ribosome binding site
poly(A) signal
4180 .. 4185  =  6 bp
poly(A) signal
4180 .. 4185  =  6 bp
ATG
2333 .. 2335  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
ATG
2333 .. 2335  =  3 bp
1 amino acid  =  149.2 Da
Product: start codon
enterokinase site
2462 .. 2476  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
enterokinase site
2462 .. 2476  =  15 bp
5 amino acids  =  606.5 Da
Product: enterokinase recognition and cleavage site
Try SnapGene and create your own beautiful maps

Individual Sequences & Maps

SnapGene offers the fastest and easiest way to plan, visualize, and document your molecular biology procedures.

Priced accessibly so that everyone in your lab can have a license.

Learn More...

SnapGene Viewer is a versatile tool for creating and sharing richly annotated sequence files. It opens many common file formats.

Free! Because there should be no barriers to seeing your data.

Learn More...

The map, notes, and annotations on this page and in the sequence/map file are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as "www.snapgene.com/resources". Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Copyright © 2016 GSL Biotech LLC | Site Map | Privacy | Legal Disclaimers   Subscribe to Our Newsletter